Allantoin in human urine quantified by ultra-performance liquid chromatography–tandem mass spectrometry

Uric acid is a potent antioxidant and scavenger of singlet oxygen and other radicals in humans. Allantoin, the predominant product of free radical-induced oxidation of uric acid, is efficiently excreted in the urine and has potential as a biomarker of oxidative stress. We developed a rapid and speci...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 402; no. 2; pp. 191 - 193
Main Authors Tolun, Adviye A., Zhang, Haoyue, Il’yasova, Dora, Sztáray, Judit, Young, Sarah P., Millington, David S.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 15.07.2010
Subjects
Adolescent
Adult
Aged
Allantoin - urine
Child
Child, Preschool
Chromatography, High Pressure Liquid - methods
Female
Humans
Limit of Detection
Male
Middle Aged
Tandem Mass Spectrometry - economics
Tandem Mass Spectrometry - methods
Young Adult
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Summary:Uric acid is a potent antioxidant and scavenger of singlet oxygen and other radicals in humans. Allantoin, the predominant product of free radical-induced oxidation of uric acid, is efficiently excreted in the urine and has potential as a biomarker of oxidative stress. We developed a rapid and specific assay for urinary allantoin using ultra-performance liquid chromatography–tandem mass spectrometry suitable for high-throughput clinical studies. The method required minimal sample preparation and was accurate (mean error=6%), precise (intra- and interday imprecision <8%), and sensitive (limit of detection=0.06pmol). Allantoin levels measured in control samples were comparable to literature values.
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ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2010.03.033