Transfer of high-mannose-type oligosaccharides to disaccharides by endo-β- N-acetylglucosaminidase from Arthrobacter protophormiae

• Published in: Journal of bioscience and bioengineering Vol. 93; no. 6; pp. 614 - 617
• Main Authors: Fujita, Kiyotaka, Miyamura, Tsuyoshi, Sano, Mutsumi, Kato, Ikunoshin, Takegawa, Kaoru
• Format: Journal Article
• Language: English
• Published: Amsterdarm Elsevier B.V 2002; Elsevier Science
• Subjects: anomeric configuration; Biological and medical sciences; Biotechnology; disaccharide; endo-β- N-acetylglucosaminidase; Enzyme engineering; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Miscellaneous; transglycosylation; endo-β- N-acetylglucosaminidase; anomeric configuration; transglycosylation; disaccharide; Enzyme; Enzymatic catalysis; Mannose; Disaccharide; Substrate; Transglycosylation; Mannosyl-glycoprotein endo-β-N-acetylglucosamidase; Enzymatic activity; Glycosidases; Bacteria; Hydrolases; Actinomycetes; Glycoside; O-Glycosidases; Arthrobacter
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/S1389-1723(02)80247-X

Endo-β- N-acetylglucosaminidase from Arthrobacter protophormiae (Endo-A) has transglycosylation activity, and high-mannose-type oligosaccharides are transferred to suitable glycosides as acceptor substrates. The acceptor specificity of Endo-A-catalyzed transglycosylation toward various disaccharides was investigated. To identify an effective acceptor for the transglycosylation by Endo-A, the reaction was carried out using various disaccharides. Endo-A transferred high-mannose-type oligosaccharides more efficiently to β-linked disaccharides (cellobiose, gentiobiose, sophorose, and laminaribiose) than to α-linked disaccharides (isomaltose, maltose, nigerose, kojibiose, and trehalose) as acceptor substrates. The transglycosylation products, (Man) 6GlcNAc-Glc-β-Glc, were more rapidly hydrolyzed than (Man) 6GlcNAc-Glc-α-Glc. These results indicate that Endo-A recognizes the anomeric configuration of the acceptor substrates, and β-linked glycosides are suitable for the synthesis of transglycosylation products.