Assessment of the antibacterial activity of phenylethanoid glycosides from Phlomis lanceolata against multiple-drug-resistant strains of Staphylococcus aureus

• Published in: Journal of natural medicines Vol. 62; no. 1; pp. 91 - 95
• Main Authors: Nazemiyeh, Hossein, Rahman, M. Mukhlesur, Gibbons, Simon, Nahar, Lutfun, Delazar, Abbas, Ghahramani, Mohammed-Ali, Talebpour, Amir-Hossein, Sarker, Satyajit D.
• Format: Journal Article
• Language: English
• Published: Tokyo Springer-Verlag 01.01.2008
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Biomedical and Life Sciences; Biomedicine; Chromatography, High Pressure Liquid; Complementary & Alternative Medicine; Drug Resistance, Multiple, Bacterial; Glucosides - pharmacology; Glycosides - chemistry; Glycosides - isolation & purification; Glycosides - pharmacology; Magnetic Resonance Spectroscopy; Mass Spectrometry; Medicinal Chemistry; Microbial Sensitivity Tests; Molecular Structure; Pharmacology/Toxicology; Pharmacy; Phenols - pharmacology; Phlomis - chemistry; Plant Leaves; Plant Sciences; Spectrophotometry, Ultraviolet; Staphylococcus aureus - drug effects; Staphylococcus aureus - growth & development; Anti-MRSA activity; Forsythoside B; Phlinoside C; Lamiaceae; Verbascoside; Phenylethanoid glycoside
• ISSN: 1340-3443; 1861-0293
• DOI: 10.1007/s11418-007-0194-z

Three phenylethanoid glycosides, forsythoside B ( 1 ), phlinoside C ( 2 ) and verbascoside ( 3 ), were isolated from the methanol extract of the leaves of Phlomis lanceolata , an Iranian medicinal plant, by reversed-phase preparative high-performance liquid chromatography (HPLC), and the structures of these compounds were elucidated conclusively by ultraviolet (UV), mass spectrometry (MS) and a series of 1D and 2D nuclear magnetic resonance (NMR) analyses. The antibacterial properties of 1 – 3 against five multi-drug-resistant (MDR) strains of Staphylococcus aureus have been assessed by the rapid and robust microtitre-plate-based serial dilution method. While compounds 1 and 3 showed considerable activities against all five strains, compound 2 was inactive at the test concentrations.