Respiratory consequences of red sludge dust inhalation in rats

• Published in: Toxicology letters Vol. 209; no. 2; pp. 113 - 120
• Main Authors: Czövek, Dorottya, Novák, Zoltán, Somlai, Csaba, Asztalos, Tibor, Tiszlavicz, László, Bozóki, Zoltán, Ajtai, Tibor, Utry, Noémi, Filep, Ágnes, Bari, Ferenc, Peták, Ferenc
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 07.03.2012; Elsevier
• Subjects: Airway resistance; Alumina plant; Animals; Biological and medical sciences; Bronchial hyper-reactivity; Bronchial Hyperreactivity - chemically induced; Bronchial Hyperreactivity - immunology; Dust; Fugitive dust; Histocytochemistry; Hungary; Inhalation Exposure - adverse effects; Lung - cytology; Lung - drug effects; Lung - immunology; Lung - pathology; Lung compliance; Lung function; Macrophages, Alveolar - drug effects; Macrophages, Alveolar - immunology; Macrophages, Alveolar - pathology; Male; Medical sciences; Particle Size; Particulate Matter - toxicity; Rats; Rats, Wistar; Sewage; Toxicology; Hungary; Europe; Airway resistance; Lung compliance; Lung function; Bronchial hyper-reactivity; Alumina plant; Fugitive dust; Hyperreactivity; Rat; Lung; Rodentia; Bronchus; Disaster; Respiratory system; Inhalation; Resistance; Respiratory tract; Vertebrata; Dust; Mammalia; Flood; Animal; Alumina
• ISSN: 0378-4274; 1879-3169
• DOI: 10.1016/j.toxlet.2011.12.006

► We characterize the pulmonary consequences of a prolonged red sludge dust inhalation. ► We explore mechanisms of subsequent airway hyper-responsiveness (AH) in rats. ► We find no adverse changes in basal lung function. ► AH and airway inflammation developed. ► In conclusion, no greater respiratory hazard is expected than urban dust inhalation. The environmental disaster following flooding by red sludge in the Ajka region in Hungary poses a serious public health threat with particular concern regarding the potentially adverse respiratory effects of the inhalation of red sludge dust (RSD). The respiratory consequences of the inhalation of RSD obtained from field samples were investigated in rats. Rats were either exposed to RSD at a high concentration (2 weeks, 8h/day), or kept in room air. After the exposures, the airway resistance (Raw) and the respiratory tissues mechanics were measured under baseline condition, and following methacholine (MCh) challenges with the aim of establishing airway hyper-responsiveness (AH). Histopathology was performed to assess lung morphologic alterations. The physical properties and the chemical composition of the RSD were also characterized. The size distribution, chemical composition and topology of the RSD particles applied in our experiments were similar to those observed at the site of the disaster. The inhalation of RSD did not alter the basal respiratory mechanics, whereas it led to greater MCh-induced responses in Raw, demonstrating the progression of mild AH. Histopathological investigations revealed fine, granular particles in the alveolar macrophages, as evidence that RSD had reached the lower respiratory tract and induced mild inflammation around the alveoli and the pulmonary vasculature. The mild respiratory symptoms that developed following short-term exposure of healthy individuals to high concentrations of airborne RSD do not appear to pose a greater respiratory hazard than the inhalation of urban dust at a comparable concentration.