Intestinal oxygen uptake and glucose metabolism during nutrient absorption in the pig
Intestinal transport of nutrients coincides with their partial catabolism in the gut. The aim of the present study was to measure intestinal oxygen consumption and nutrient metabolism after a meal or during a short fast. Nutrient and oxygen balances aeross the small intestine were measured in consci...
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Published in | Proceedings of the Society for Experimental Biology and Medicine Vol. 207; no. 3; p. 309 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.12.1994
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Subjects | |
Online Access | Get more information |
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Summary: | Intestinal transport of nutrients coincides with their partial catabolism in the gut. The aim of the present study was to measure intestinal oxygen consumption and nutrient metabolism after a meal or during a short fast. Nutrient and oxygen balances aeross the small intestine were measured in conscious 50 kg (live wt) pigs. Jejunal enterocytes were also isolated from 1-hr postprandial, postabsorptive, or 3 day fasted pigs, in order to evaluate their capacities to metabolize 5 mM glucose and 2 mM glutamine. Whatever the nutritional state, intestinal oxygen consumption was high, since 26 +/- 2% (n = 6) of the oxygen arterial supply was extracted by the small intestine. Furthermore, the consumption of a mixed meal induced a rapid and transient rise in oxygen consumption in the postabsorptive state, the intestinal uptake of glucose (0.31 +/- 0.08 mmole/min, n = 6) was twice higher than that of glutamine. The role of glucose as a fuel was also evidenced after a 3-day fast. During nutrient absorption, glutamine was highly utilized, and lactate was produced. The capacity of enterocytes isolated from fed pigs to metabolize glucose was dramatically reduced, as was 6-phosphofructo 1-kinase activity. In contrast, intestinal muscle presented a high glycolytic capacity from glucose, suggesting that the main diet of intestinal lactate production during nutrient absorption would be the muscular rather than the epithelial layer |
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Bibliography: | S20 9537731 |
ISSN: | 0037-9727 1525-1373 |
DOI: | 10.3181/00379727-207-43821 |