Intestinal oxygen uptake and glucose metabolism during nutrient absorption in the pig

• Published in: Proceedings of the Society for Experimental Biology and Medicine Vol. 207; no. 3; p. 309
• Main Authors: Vaugelade, P. (INRA, UEPSD, Jouy-en-Josas, France), Posho, L, Darcy-Vrillon, B, Bernard, F, Morel, M.T, Duee, P.H
• Format: Journal Article
• Language: English
• Published: United States 01.12.1994
• Subjects: ABSORCION DIGESTIVA; ABSORPTION DIGESTIVE; ACIDE LACTIQUE; ACIDO LACTICO; ALANINA; ALANINE; Animals; CATABOLISME; CATABOLISMO; CELLULE; CELULAS; CERDO; CONSOMMATION D'OXYGENE; CONSUMO DE OXIGENO; ESTADO NUTRICIONAL; ETAT NUTRITIONNEL; Female; GLUCOSA; GLUCOSE; Glucose - metabolism; GLUTAMINA; GLUTAMINE; Glutamine - metabolism; Glycolysis; Insulin - blood; INTESTIN; Intestinal Absorption; Intestinal Mucosa - metabolism; INTESTINOS; Lactates - biosynthesis; Lactic Acid; METABOLISME DES GLUCIDES; METABOLISMO DE CARBOHIDRATOS; MODELE; MODELOS; Oxygen Consumption; PORCIN; Swine
• ISSN: 0037-9727; 1525-1373
• DOI: 10.3181/00379727-207-43821

Intestinal transport of nutrients coincides with their partial catabolism in the gut. The aim of the present study was to measure intestinal oxygen consumption and nutrient metabolism after a meal or during a short fast. Nutrient and oxygen balances aeross the small intestine were measured in conscious 50 kg (live wt) pigs. Jejunal enterocytes were also isolated from 1-hr postprandial, postabsorptive, or 3 day fasted pigs, in order to evaluate their capacities to metabolize 5 mM glucose and 2 mM glutamine. Whatever the nutritional state, intestinal oxygen consumption was high, since 26 +/- 2% (n = 6) of the oxygen arterial supply was extracted by the small intestine. Furthermore, the consumption of a mixed meal induced a rapid and transient rise in oxygen consumption in the postabsorptive state, the intestinal uptake of glucose (0.31 +/- 0.08 mmole/min, n = 6) was twice higher than that of glutamine. The role of glucose as a fuel was also evidenced after a 3-day fast. During nutrient absorption, glutamine was highly utilized, and lactate was produced. The capacity of enterocytes isolated from fed pigs to metabolize glucose was dramatically reduced, as was 6-phosphofructo 1-kinase activity. In contrast, intestinal muscle presented a high glycolytic capacity from glucose, suggesting that the main diet of intestinal lactate production during nutrient absorption would be the muscular rather than the epithelial layer