An Aptamer Biosensing Strategy for Label-Free Assay of Dual Acute Myocardial Infarction Biomarkers Built upon AuNPs/Ti3C2-MXenes

• Published in: Chemosensors Vol. 11; no. 3; p. 157
• Main Authors: Mi, Xiaona, Li, Huiling, Tu, Yifeng
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.03.2023
• Subjects: acute myocardial infarction; aptamer; Aptamers; AuNPs/Ti3C2-MXenes; Biomarkers; Biosensors; Calcium-binding protein; cardiac troponin I; Diagnosis; electrochemical biosensor; Electrodes; Ferricyanide; Gold; Heart attacks; Indium tin oxides; Iron; Microscopy; MXenes; Myocardial infarction; myoglobin; Myoglobins; Potassium; Self-assembly; Sensors; Spectrum analysis; Titanium; Troponin I; Voltammetry; China
• ISSN: 2227-9040; 2227-9040
• DOI: 10.3390/chemosensors11030157

The sensitive quantification of cardiac troponin I (cTnI) and myoglobin (Myo) in blood is essential for an early emergency diagnosis of acute myocardial infarction (AMI). Attributed to AuNPs and a titanium element on the surface of the AuNPs/Ti3C2-MXenes hybrid, each respective aptamer strand can be immobilized on. In this work, a nanohybrid was deposited on amino-functionalized indium tin oxide (ITO) via an Au–N bond; thereafter, it could catch cTnI-specific, thiol-functionalized DNA aptamer through Au–S self-assembly or Myo-aptamer via adsorption and metal chelate interaction between phosphate groups and titanium for specific recognition. Both using [Fe(CN)6]3−/4− as a signaling probe, the differential pulse voltammetric (DPV) current of the cTnI-aptasensor decreased after binding with cTnI, while the other responded to Myo via the impedimetric measurement. These developed biosensors enable the response to the femtogram/mL level cTnI or nanogram/mL level Myo. Remarkably, the proposed aptasensors exhibit high sensitivity and specificity for targets and display great potential for applications in clinic diagnosis.