CCC1 Is a Transporter That Mediates Vacuolar Iron Storage in Yeast
The budding yeast Saccharomyces cerevisiae can grow for generations in the absence of exogenous iron, indicating a capacity to store intracellular iron. As cells can accumulate iron by endocytosis we studied iron metabolism in yeast that were defective in endocytosis. We demonstrated that endocytosi...
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Published in | The Journal of biological chemistry Vol. 276; no. 31; pp. 29515 - 29519 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Society for Biochemistry and Molecular Biology
03.08.2001
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Subjects | |
Online Access | Get full text |
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Summary: | The budding yeast Saccharomyces cerevisiae can grow for generations in the absence of exogenous iron, indicating a capacity to store intracellular iron. As cells can
accumulate iron by endocytosis we studied iron metabolism in yeast that were defective in endocytosis. We demonstrated that
endocytosis-defective yeast ( Îend4 ) can store iron in the vacuole, indicating a transfer of iron from the cytosol to the vacuole. Using several different criteria
we demonstrated that CCC1 encodes a transporter that effects the accumulation of iron and Mn 2+ in vacuoles. Overexpression of CCC1, which is localized to the vacuole , lowers cytosolic iron and increases vacuolar iron content. Conversely, deletion of CCC1 results in decreased vacuolar iron content and decreased iron stores, which affect cytosolic iron levels and cell growth.
Furthermore Îccc1 cells show increased sensitivity to external iron. The sensitivity to iron is exacerbated by ectopic expression of the iron
transporter FET4 . These results indicate that yeast can store iron in the vacuole and that CCC1 is involved in the transfer of iron from the cytosol to the vacuole. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.M103944200 |