Screening of Genetic Polymorphisms of CYP3A4 and CYP3A5 Genes

• Published in: The Korean journal of physiology & pharmacology Vol. 17; no. 6; pp. 479 - 484
• Main Authors: Lee, Jin Sol, Cheong, Hyun Sub, Kim, Lyoung Hyo, Kim, Ji On, Seo, Doo Won, Kim, Young Hoon, Chung, Myeon Woo, Han, Soon Young, Shin, Hyoung Doo
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Physiological Society and The Korean Society of Pharmacology 01.12.2013; 대한약리학회
• Subjects: Original; 약리학; Pharmacogenetics; SNP; CYP3A4; CYP3A5; Cytochrome P450
• ISSN: 1226-4512; 2093-3827
• DOI: 10.4196/kjpp.2013.17.6.479

Given the CYP3A4 and CYP3A5's impact on the efficacy of drugs, the genetic backgrounds of individuals and populations are regarded as an important factor to be considered in the prescription of personalized medicine. However, genetic studies with Korean population are relatively scarce compared to those with other populations. In this study, we aimed to identify CYP3A4/5 polymorphisms and compare the genotype distributions among five ethnicities. To identify CYP3A4/5 SNPs, we first performed direct sequencing with 288 DNA samples which consisted of 96 Koreans, 48 European-Americans, 48 African-Americans, 48 Han Chinese, and 48 Japanese. The direct sequencing identified 15 novel SNPs, as well as 42 known polymorphisms. We defined the genotype distributions, and compared the allele frequencies among five ethnicities. The results showed that minor allele frequencies of Korean population were similar with those of the Japanese and Han Chinese populations, whereas there were distinct differences from European-Americans or African-Americans. Among the pharmacogenetic markers, frequencies of CYP3A4*1B (rs2740574) and CYP3A5*3C (rs776742) in Asian groups were different from those in other populations. In addition, minor allele frequency of CYP3A4*18 (rs28371759) was the highest in Korean population. Additional in silico analysis predicted that two novel non-synonymous SNPs in CYP3A5 (+27256C>T, P389S and +31546T>G, I488S) could alter protein structure. The frequency distributions of the identified polymorphisms in the present study may contribute to the expansion of pharmacogenetic knowledge.