Therapeutic Potential of Mangosteen Pericarp Extract-Loaded Liposomes against Superficial Skin Infection Caused by Staphylococcus pseudintermedius in a Murine Model

• Published in: Antibiotics (Basel) Vol. 13; no. 7; p. 612
• Main Authors: Kim, Seong-Yeop, Park, Seong-Yong, Lee, Jung-Hwa, Kim, Nayeong, Oh, Ha-Na, Yoo, So-Young, Lee, Dae-Sung, Lee, Je-Chul
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.07.2024
• Subjects: Animal diseases; Animal models; Animals; Antibacterial activity; Antiinfectives and antibacterials; Antimicrobial agents; Bacterial infections; Bioavailability; Biocompatibility; Casein; Chromatography; companion animals; Cyclodextrin; Cyclodextrins; Cytotoxicity; Dilution; Dogs; Drug resistance in microorganisms; Garcinia mangostana; Gram-positive bacteria; Health aspects; In vivo methods and tests; Inclusion complexes; Infections; Lecithin; Lesions; Liposomes; mangosteen pericarp; Methicillin; Pericarp; Progenitor cells; Pyoderma; Skin; Skin diseases; Skin lesions; Staphylococcal infections; Staphylococcus; Staphylococcus species; superficial pyoderma; Wound healing; α-mangostin; South Korea; Staphylococcus species; α-mangostin; superficial pyoderma; mangosteen pericarp; companion animals
• ISSN: 2079-6382; 2079-6382
• DOI: 10.3390/antibiotics13070612

α-mangostin (α-MG) demonstrates antibacterial activity against species. Therefore, this study aimed to explore the antibacterial activity of α-MG-rich mangosteen pericarp extract (MPE)-loaded liposomes against isolates from companion animal skin diseases in vitro and evaluated their therapeutic potential in a murine model of superficial skin infection caused by . α-MG-rich extract was purified from mangosteen pericarp and then complexed with γ-cyclodextrin (γ-CD), forming the inclusion complexes. Nanoliposomes containing MPE and γ-CD complexes were prepared by adding lecithin and casein. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of MPE-loaded liposomes were determined using agar dilution and broth microdilution methods. The therapeutic potential of MPE-loaded liposomes was evaluated in vivo on tape-stripped skin lesions infected with . Purified MPE and MPE-loaded liposomes contained 402.43 mg/g and 18.18 mg/g α-MG, respectively. MPE-loaded liposomes showed antibacterial activity against clinical isolates in vitro but did not show antibacterial activity against Gram-negative bacterial isolates. MPE-loaded liposomes demonstrated consistent MICs and MBCs against isolates. These liposomes significantly reduced bacterial numbers and lesional sizes in a superficial skin infection model. Moreover, they reconstructed the epidermal barrier in skin lesions. The therapeutic concentrations of MPE-loaded liposomes did not induce cytotoxicity in canine progenitor epidermal keratinocyte cells. In conclusion, MPE-loaded liposomes hold promise for the development of a prospective topical formulation to treat superficial pyoderma in companion animals.