The effect of therapeutic ultrasound on repair of the achilles tendon ( tendo calcaneus) of the rat

• Published in: Ultrasound in medicine & biology Vol. 27; no. 12; pp. 1691 - 1696
• Main Authors: da Cunha, Alessandra, Parizotto, Nivaldo Antonio, Vidal, Benedicto de Campos
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.12.2001; Elsevier
• Subjects: Achilles Tendon - diagnostic imaging; Achilles Tendon - injuries; Analysis of Variance; Animals; Anisotropic properties; Biological and medical sciences; Biomechanical Phenomena; Birefringence; Collagen - biosynthesis; Collagen synthesis; Diseases of the osteoarticular system. Orthopedic treatment; Male; Medical sciences; Molecular order; Polarized light; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); Rats; Rats, Wistar; Tendon; Tendon Injuries - diagnostic imaging; Tendon Injuries - physiopathology; Tendon Injuries - therapy; Therapeutic ultrasound; Tissue repair; Type I collagen; Ultrasonic Therapy; Ultrasonography; Wound healing; Wound Healing - physiology; Collagen synthesis; Molecular order; Wound healing; Anisotropic properties; Therapeutic ultrasound; Birefringence; Type I collagen; Tissue repair; Polarized light; Tendon; Animal model; Rat; Tendo calcaneus; Treatment efficiency; Rodentia; Diseases of the osteoarticular system; Ultrasonic treatment; Experimental study; Pathology; Vertebrata; Optical microscopy; Mammalia; Anisotropy; Animal; Collagen; Tissue rupture; Cicatrization
• ISSN: 0301-5629; 1879-291X
• DOI: 10.1016/S0301-5629(01)00477-X

The purpose of this study was to evaluate the effects of therapeutic ultrasound (US) on the healing process in the Achilles tendon ( Tendo calcaneus) of Wistar rats after tenotomy. Sonication was performed at a frequency of 1 MHz, an intensity of 0.5 W/cm 2 (SATA), for 5 min, over a period of 14 consecutive days in two modalities ( n = 15); in both continuous and pulsed modes. The control group was divided into tenotomized, mock-sonicated and nontenotomized tendons ( n = 15). On the 15th postoperative day, the tendons were removed and analyzed by using the polarized light microscopy, with the purpose of detecting and measuring the organization of collagen fibers through birefringence. The results showed a high birefringence for the tendons treated using the pulsed mode ( p < 0.001), revealing the best organization and aggregation of collagen bundles. Sonication in the continuous mode induced a decrease ( p = 0.047) in the ability to quicken the healing process. These findings suggest that US therapy is beneficial in the early healing process of tendons when the pulsed mode is used. (E-mail: parizoto@power.ufscar.br)