LC determination of YM466, a new factor Xa inhibitor, in rat and dog plasma

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 29; no. 4; pp. 631 - 638
• Main Authors: Mano, Yuji, Aoki, Toshiko, Kikuchi, Yumiko, Soeishi, Yoshiaki, Usui, Takashi, Kamimura, Hidetaka
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 20.07.2002; Elsevier Science
• Subjects: Analysis; Analytical method; Animals; Anticoagulants - blood; Biological and medical sciences; Chromatography, High Pressure Liquid; Dogs; Drug Stability; Factor Xa inhibitor; Factor Xa Inhibitors; Freezing; General pharmacology; Male; Medical sciences; Naphthalenes - blood; Pharmacology. Drug treatments; Piperidines - blood; Plasma; Rats; Rats, Inbred F344; Sensitivity and Specificity; Spectrophotometry, Ultraviolet; YM466; Rats; Plasma; YM466; Analytical method; Factor Xa inhibitor; Dogs; Fissipedia; Solid phase extraction; Biological fluid; Carnivora; Serine endopeptidases; Rat; Enzyme; Rodentia; Oral administration; HPLC chromatography; Coagulation Factor Xa; Anticoagulant; Blood plasma; Peptidases; Vertebrata; Mammalia; Ultraviolet detector; Analysis method; Animal; Hydrolases; Inhibitor; Pharmacokinetics; Dog; Quantitative analysis
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/S0731-7085(02)00103-6

A specific method for the determination of YM466, a novel Factor Xa inhibitor, in rat and dog plasma was developed and validated. YM466 was extracted from plasma by solid-phase extraction and analyzed by UV-HPLC at an absorbance wavelength of 240 nm. The intra-day precision and accuracy ranged from 0.8 to 2.4% and 0.1 to 5.0% in rats, and from 1.6 to 2.4% and 0.0 to 4.1% in dogs, respectively. The lower limit of quantification was 10 ng/ml when 1 ml of plasma was used. No endogenous interference was observed in the plasma of rats and dogs.