Electrochemical Dissolved Oxygen Sensor-Integrated Platform for Wireless In Situ Bioprocess Monitoring

•Integrated wireless platform for real-time, autonomous, in situ electrochemical monitoring of chemical analytes in bioreactors.•Dissolved oxygen is electrochemically detected by a tape-based Clark-type sensor•Entire free-floating system is implemented in a 3-D printed shell including the sensor, su...

Full description

Saved in:
Bibliographic Details
Published inSensors and actuators. B, Chemical Vol. 320; p. 128381
Main Authors Stine, Justin M., Beardslee, Luke A., Sathyam, Rajendra M., Bentley, William E., Ghodssi, Reza
Format Journal Article
LanguageEnglish
Published Lausanne Elsevier B.V 01.10.2020
Elsevier Science Ltd
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:•Integrated wireless platform for real-time, autonomous, in situ electrochemical monitoring of chemical analytes in bioreactors.•Dissolved oxygen is electrochemically detected by a tape-based Clark-type sensor•Entire free-floating system is implemented in a 3-D printed shell including the sensor, supporting electronics, a battery and a Bluetooth Low Energy wireless link for data transmission.•Potential applications in bioprocess monitoring in large-scale industrial bioreactors. This work presents a bio-processing online device (bPod) platform, capable of real-time in situ monitoring of bioreactor cell culture parameters, such as dissolved oxygen (DO). The bPod is an integrated system comprised of a potentiostat analog-front-end (AFE), a Bluetooth Low Energy (BLE) microcontroller, and a chemical sensor, exemplified here by a Clark-type DO sensor, which enables monitoring of dissolved oxygen content. The Clark-type electrochemical sensor performs chronoamperometric measurement of DO percent saturation, and the BLE microcontroller wirelessly transmits data to a smartphone while submerged in aqueous media. After several electrode design modifications, the bPod showed a linear electrochemical current response corresponding to DO percent saturation levels with a sensitivity of 37.5 nA/DO% and limit of detection of 8.26 DO%, covering concentration ranges relevant for mammalian culture processes within bioreactors. The wireless bPod provides a free-floating capsule architecture for monitoring DO and can be adapted for an array of electrochemical sensors, targeting different process parameters for diverse bioprocess monitoring applications.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2020.128381