Inhibition of the Splicing of Glucose-6-phosphate Dehydrogenase Precursor mRNA by Polyunsaturated Fatty Acids

• Published in: The Journal of biological chemistry Vol. 277; no. 34; pp. 31270 - 31278
• Main Authors: Tao, Huimin, Szeszel-Fedorowicz, Wioletta, Amir-Ahmady, Batoul, Gibson, Matthew A, Stabile, Laura P, Salati, Lisa M
• Format: Journal Article
• Language: English
• Published: United States American Society for Biochemistry and Molecular Biology 23.08.2002
• Subjects: Animals; Arachidonic Acid - pharmacology; Cells, Cultured; Fatty Acids, Unsaturated - pharmacology; Gene Expression Regulation - drug effects; Glucosephosphate Dehydrogenase - genetics; Hepatocytes - metabolism; Introns; Male; Mice; Mice, Inbred C57BL; Promoter Regions, Genetic; Rats; Rats, Sprague-Dawley; RNA Precursors - genetics; RNA Splicing - drug effects

Polyunsaturated fatty acids inhibit the expression of hepatic glucose-6-phosphate dehydrogenase (G6PD) by changes in the amount of G6PD pre-mRNA in the nucleus in the absence of changes in the transcription rate of the gene. We have compared the nuclear accumulation of partially and fully spliced mR...