Fully automated on-line quantification of quetiapine in human serum by solid phase extraction and liquid chromatography

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 798; no. 1; pp. 9 - 16
• Main Authors: Hasselstrøm, J, Linnet, K
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 05.12.2003; Elsevier Science
• Subjects: Analysis; Antipsychotic Agents - blood; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Dibenzothiazepines - blood; General pharmacology; Humans; Medical sciences; Pharmacology. Drug treatments; Quetiapine; Quetiapine Fumarate; Reproducibility of Results; Sensitivity and Specificity; Spectrophotometry, Ultraviolet; Quetiapine; Human; Drug; Solid phase extraction; Biological fluid; Trifluoperazine; Neuroleptic; Psychotropic; HPLC chromatography; Hypnotic; Liquid chromatography; Phenothiazine derivatives; Therapeutic drug monitoring; Tranquillizer; Analysis method; Serum; Quantitative analysis
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/S1570-0232(03)00592-0

A quantitative method for determination of quetiapine (QTP) in human serum is presented. The method is fully automated and based on high performance liquid chromatography (HPLC) with on-line solid phase extraction (SPE). The extraction procedure is based on a C2 cartridge, which is eluted with methanol. The eluate is injected onto a silica column with a mobile phase consisting of methanol:20 mM NH 4CH 3COO, pH 5.0 (99:1). Quetiapine is quantified by ultra-violet (UV) absorbance at 257 nM with trifluoperazine as the internal standard (I.S.). The extraction recoveries for quetiapine and trifluoperazine were 69 and 57%, respectively. The total inter day coefficient of variation was 11.1, 3.8 and 3.1% at 20, 500 and 1000 nM, respectively. The detection limit was 10.3 nM quetiapine. The method has been used in our therapeutic drug monitoring (TDM) laboratory where co-administered drugs often are observed. In an investigation of analytical interference from co-administered drugs, demethyl-mianserine was the only drug which interfered with the internal standard. There was no interference with quetiapine itself. The method showed good agreement with mass spectrometric quantification of quetiapine.