Increased concentration of thymidine kinase in rat hepatomas

Thymidine kinase was purified to near homogeneity by affinity chromatography from cytosol fraction of rat hepatoma 3924A. The enzyme had a Mr of 81,000 and was composed of two subunits of Mr = 44,000. Antiserum made against it neutralized the activities of thymidine kinase from both rat livers and h...

Full description

Saved in:
Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 111; no. 1; pp. 280 - 287
Main Authors Lai, Mei-Huei T., Weber, George
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 28.02.1983
Subjects
Animals
Chromatography, Affinity
cytosol
hepatoma
Liver - enzymology
Liver Neoplasms, Experimental - enzymology
Macromolecular Substances
Male
Molecular Weight
Rats
Rats, Inbred BUF
thymidine kinase
Thymidine Kinase - analysis
Thymidine Kinase - metabolism
Online AccessGet full text

Cover

More Information
Summary:Thymidine kinase was purified to near homogeneity by affinity chromatography from cytosol fraction of rat hepatoma 3924A. The enzyme had a Mr of 81,000 and was composed of two subunits of Mr = 44,000. Antiserum made against it neutralized the activities of thymidine kinase from both rat livers and hepatomas. Neutralization studies with the antiserum revealed that hepatic transformation resulted in 4-, 15- and 25-fold increase in the amount of cytosol thymidine kinase in hepatomas 16, 7787 and 3924A of slow, medium and fast growth rate, respectively.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(83)80148-X