Usefulness and limitations of various guinea-pig test methods in detecting human skin sensitizers—Validation of guinea-pig tests for skin hypersensitivity

• Published in: Food and chemical toxicology Vol. 20; no. 1; pp. 67 - 74
• Main Authors: Marzulli, F., Maguire, H.C.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.1982
• Subjects: Allergens - toxicity; Animals; Dermatitis, Contact; Drug Evaluation, Preclinical - methods; Female; Guinea Pigs; Humans; GPMT; DMSO; CFA
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/S0278-6915(82)80011-2

Several guinea-pig predictive test methods were evaluated by comparison of results with those obtained with human predictive tests, using ten compounds that have been used in cosmetics. The method involves the statistical analysis of the frequency with which guinea-pig tests agree with the findings of tests in humans. In addition, the frequencies of false positive and false negative predictive findings are considered and statistically analysed. The results clearly demonstrate the superiority of adjuvant tests (complete Freund's adjuvant) in determining skin sensitizers and the overall superiority of the guinea-pig maximization test in providing results similar to those obtained by human testing. A procedure is suggested for utilizing adjuvant and non-adjuvant test methods for characterizing compounds as of weak, moderate or strong sensitizing potential.