Genome-Wide Identification and Expression Analysis of CAMTA Gene Family Implies PbrCAMTA2 Involved in Fruit Softening in Pear

CAMTA are calcium-modulating binding transcription factors that contribute to plant development. We identified 46 CAMTA genes from eight Rosaceae species and divided them into five subgroups based on a phylogenetic tree. Our analysis indicated that CAMTA is a highly conserved family among Rosaceae s...

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Bibliographic Details
Published inHorticulturae Vol. 9; no. 4; p. 467
Main Authors Yu, Jinshan, Song, Bobo, Gu, Kaidi, Cao, Beibei, Zhao, Kejiao, Wu, Jun, Li, Jiaming
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.04.2023
Subjects
Binding
CAMTA
Chromosomes
Deoxyribonucleic acid
DNA
Domains
fruit softening
Fruits
Gene expression
Gene families
Genes
Genomes
Geographical distribution
Metabolism
Ovaries
pear
Phylogenetics
Phylogeny
Proteins
qRT-PCR
Regulatory sequences
Rosaceae
Softening
Software
Subgroups
synteny analysis
Transcription factors
Zein
Beijing China
China
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Summary:CAMTA are calcium-modulating binding transcription factors that contribute to plant development. We identified 46 CAMTA genes from eight Rosaceae species and divided them into five subgroups based on a phylogenetic tree. Our analysis indicated that CAMTA is a highly conserved family among Rosaceae species, with a conserved DNA-binding domain (CG-1) and a conserved transcription factor immunoglobulin domain (TIG). Following a recent whole-genome duplication event, the genomes of Chinese white pear, European pear, and apple experienced significant expansion, resulting in the number of CAMTA genes being twice that of the other species. Cis-element identification showed that the distribution of the zein metabolism regulation-responsive element was different in the promoters of Chinese white pear (55.56%) and European pear (11.11%) CAMTA gene families. The gene expression results showed that PbrCAMTA1, 2, 6, 7 was highly expressed in pear fruit. Among them, PbrCAMTA2 may have a key influence on fruit softening, as observed in transient transformation experiments. In conclusion, our results provide crucial insights into the evolution of the CAMTA gene family in pear and other Rosaceae species and identify a candidate PbrCAMTA gene, which is involved in the dynamic development of pear fruits.
ISSN:2311-7524
2311-7524
DOI:10.3390/horticulturae9040467