An in vitro mucosal model for prediction of the bioadhesion of alginate solutions to the oesophagus

• Published in: International journal of pharmaceutics Vol. 238; no. 1; pp. 123 - 132
• Main Authors: Batchelor, H.K, Banning, D, Dettmar, P.W, Hampson, F.C, Jolliffe, I.G, Craig, D.Q.M
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.05.2002; Elsevier
• Subjects: Adhesiveness; Adhesives; Alginate; Alginates - chemistry; Animals; Bioadhesion; Biological and medical sciences; Esophagus; Excipients; Fluorescent Dyes; General pharmacology; In Vitro Techniques; Medical sciences; Models, Biological; Mucoadhesion; Mucous Membrane; Oesophagus; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Polymers; Predictive Value of Tests; Swine; Bioadhesion; Alginate; Oesophagus; Mucoadhesion; Viscosity; Pharmaceutical technology; Digestive system; Mucosa; Prediction; Alginates; Adhesion; In vitro; Molecular mass; Bioadhesive system; Esophagus
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/S0378-5173(02)00062-5

This paper discusses the development of an in vitro model utilised to assess the adhesion of alginate solutions to porcine oesophageal tissue. The methodology involved the construction of retention apparatus onto which sections of tissue were mounted. Fluorescently labelled alginate solutions of known rheological profile were dispensed onto the tissue at a concentration of 2% w/v. A washing solution was applied at a specified rate to mimic saliva flow and the eluted material collected. Fluorimetric analysis allowed dose retention to be assessed as a function of time. The effect of the nature of the washing solution and the choice of alginate were investigated. It was found that after 30 min up to 20% of the applied alginate dose remained associated with the tissue, regardless of the alginate selected from the range examined. The nature of the washing medium did not have a significant effect on retention, irrespective of the inherent mucin concentration. Overall this study indicated that the technique presented offers a viable means of studying bioadhesion of liquids and also demonstrates that alginate solutions may have an application as bioadhesive agents for localisation within the oesophagus.