Multifunctional-separation-mode ion chromatography method for determining major metabolites during multiple parallel fermentation of rice wine

• Published in: Analytical Methods Vol. 16; no. 25; pp. 4045 - 4053
• Main Authors: Hashigami, Atsushi, Tamura, Ryousei, Takezaki, Chihiro, Asano, Tohru, Yoshinaka, Taichi, Hirano, Kentarou, Takemura, Akihiko, Yamashita, Hideyuki, Nose, Akira, Kozaki, Daisuke
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry (RSC) 27.06.2024; Royal Society of Chemistry
• Subjects: Chromatography, Ion Exchange - methods; Citric Acid Cycle; Disaccharides; Fermentation; Gas chromatography; Glycolysis; Instrumentation; Ketoglutaric acid; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Metabolites; Monitoring; Oligosaccharides; Organic acids; Oryza - chemistry; Oryza - metabolism; Phthalic acid; Pyruvic acid; Saccharification; Separation; Tricarboxylic acid cycle; Wine; Wine - analysis; Wines
• ISSN: 1759-9660; 1759-9679; 1759-9679
• DOI: 10.1039/d4ay00591k

Facile and effective analysis methods are desirable for elucidating the behaviours of metabolites during fermentation reactions. Herein, a multifunctional-separation-mode ion chromatography (MFS-IC) method was developed for the simultaneous monitoring of major metabolites during multiple parallel fermentation, including those related to central carbon metabolism (saccharification, glycolysis, alcoholic fermentation, and the tricarboxylic acid (TCA) cycle). The use of two types of sulfo-modified size-exclusion columns and phthalic acid as the eluent allowed the separation of oligosaccharides (disaccharides, trisaccharides, and tetrasaccharides), glucose, pyruvate, and major organic acids during the TCA cycle ( cis -aconitate, citrate, iso -citrate, malate, fumarate, and succinate but not α-ketoglutarate) from other non-target analytes. The MFS-IC method was successfully applied to monitoring the major metabolites in the rice wine brewing process. This approach can contribute to an improved understanding of metabolite behaviour during fermentation without requiring the use of expensive advanced instrumentation methods such as liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry. Facile and effective analysis methods are desirable for elucidating the behaviours of metabolites during fermentation reactions.