A sensitive and accurate method for amino acid analysis using isotopic double labeling with fluorodinitrobenzene

A double-labeling procedure for amino acid analysis using 3H-labeled 1-fluoro-2,4-dinitrobenzene and 14C-labeled amino acids as internal standards is described. The procedure was tested by analyzing lysozyme and insulin B chain, and the results obtained were in good agreement with their accepted ami...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 105; no. 1; pp. 319 - 327
Main Authors Humphries, Martyn, Stuart Thompson, J.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.07.1980
Subjects
Amino Acids - analysis
Animals
Carbon Radioisotopes
Cattle
Chickens
Chromatography, Thin Layer
Dinitrofluorobenzene
Insulin
Isotope Labeling - methods
Muramidase
Peptide Fragments
Scintillation Counting
Tritium
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Summary:A double-labeling procedure for amino acid analysis using 3H-labeled 1-fluoro-2,4-dinitrobenzene and 14C-labeled amino acids as internal standards is described. The procedure was tested by analyzing lysozyme and insulin B chain, and the results obtained were in good agreement with their accepted amino acid compositions. Analysis of samples containing from 100 pmol of each amino acid can be achieved at an accuracy comparable to that obtained by conventional automated amino acid analysis methods, many of which require considerably more material. An important advantage is that amino acids present in low molar proportions can be separated and measured more readily than by column chromatography.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(80)90464-9