Thermostabilization of lactate oxidase by random mutagenesis

A lactate oxidase (LOD) gene from Aerococcus viridans was cloned and sequenced to generate thermostable LOD. One mutant LOD selected from a set of variants created by random mutagenesis had a half life of 6.2 min at 65 degree C, approximately three times longer than that of the wild type LOD. This m...

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Bibliographic Details
Published inBiotechnology letters Vol. 17; no. 9; pp. 975 - 980
Main Authors MINAGAWA, H, NAKAYAMA, N, NAKAMOTO, S
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 01.09.1995
Subjects
Aerococcus viridans
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Miscellaneous
Synthetic digonucleotides and genes. Sequencing
Aerococcus viridans
Lactate 2-monooxygenase
Nucleotide sequence
Enzyme
Thermal stability
Polymerase chain reaction
Streptococcaceae
Enzymatic activity
Mutagenesis
Point mutation
Bacteria
Micrococcales
Isolation
Thermostable
Oxidoreductases
Kinetic parameter
Molecular cloning
Aminoacid sequence
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Summary:A lactate oxidase (LOD) gene from Aerococcus viridans was cloned and sequenced to generate thermostable LOD. One mutant LOD selected from a set of variants created by random mutagenesis had a half life of 6.2 min at 65 degree C, approximately three times longer than that of the wild type LOD. This mutant exhibited an Asn to Asp point mutation at position 212 in the amino acid sequence.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0141-5492
1573-6776
DOI:10.1007/bf00127437