Bioelectroanalytical Determination of Rutin Based on bi-Enzymatic Sensor Containing Iridium Nanoparticles in Ionic Liquid Phase Supported in Clay

• Published in: Electroanalysis (New York, N.Y.) Vol. 23; no. 3; pp. 764 - 776
• Main Authors: Zapp, Eduardo, Brondani, Daniela, Vieira, Iolanda C., Dupont, Jairton, Scheeren, Carla W.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.03.2011; WILEY‐VCH Verlag
• Subjects: bi-Enzymatic biosensor; Clay (material); Ionic liquid; Ionic liquids; Iridium; Iridium nanoparticles; Laccase; Mathematical analysis; Montmorillonite; Nanoparticles; Polyphenol oxidase; Rutin; Voltammetry
• ISSN: 1040-0397; 1521-4109; 1521-4109
• DOI: 10.1002/elan.201000619

A matrix comprising iridium nanoparticles and 1‐butyl‐3‐methylimidazolium tetrafluoroborate ionic liquid (Ir‐BMI.BF4) supported in montmorillonite (MMT) was obtained through an efficient incorporation process. This modified clay matrix (Ir‐BMI.BF4‐MMT) was used for the immobilization of the enzymes laccase (LAC) and polyphenol oxidase (PPO) and employed in the construction of a bi‐enzymatic biosensor for determination of rutin by square‐wave voltammetry. Under optimized conditions, the analytical curve showed a linear range for rutin concentrations from 9.17×10−8 to 3.10×10−6 mol L−1 with a detection limit of 3.09×10−8 mol L−1. The method was successfully applied to the determination of rutin content in pharmaceutical samples.