In situ detection of programmed cell death in Nicotiana tabacum leaves during senescence

Leaf senescence is a highly regulated developmental process, during which cell constituents are dismantled in an ordered progression. The final death phase of senescence is a type of programmed cell death (PCD). We showed DNA fragmentation characteristic for PCD by terminal deoxynucleotidyl transfer...

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Bibliographic Details
Published inJournal of microscopy (Oxford) Vol. 230; no. 1; pp. 1 - 3
Main Authors UZELAC, B, JANOŠEVIĆ, D, BUDIMIR, S
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.04.2008
Subjects
Aging
Apoptosis
DNA Fragmentation
DNA, Plant - metabolism
In Situ Nick-End Labeling
leaf senescence
Microscopy, Fluorescence
Nicotiana - physiology
Nicotiana tabacum
Plant Leaves - physiology
programmed cell death
Staining and Labeling - methods
TUNEL assay
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Summary:Leaf senescence is a highly regulated developmental process, during which cell constituents are dismantled in an ordered progression. The final death phase of senescence is a type of programmed cell death (PCD). We showed DNA fragmentation characteristic for PCD by terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) assay not only in senescing leaves but also in young, still developing tobacco leaves.
Bibliography:http://dx.doi.org/10.1111/j.1365-2818.2008.01947.x
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-2720
1365-2818
DOI:10.1111/j.1365-2818.2008.01947.x