In Vivo Hypertensive Arterial Wall Uptake of Radiolabeled Liposomes

• Published in: Hypertension (Dallas, Tex. 1979) Vol. 15; no. 6, Part 1; pp. 600 - 605
• Main Authors: Hodis, Howard N, Amartey, John K, Crawford, Donald W, Wickham, Emily, Blankenhorn, David H
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Heart Association, Inc 01.06.1990; Hagerstown, MD Lippincott
• Subjects: ACETIC ACID; ANIMALS; AORTA; Aorta - metabolism; Aorta - pathology; Arterial hypertension. Arterial hypotension; ARTERIES; BASIC BIOLOGICAL SCIENCES; BETA DECAY RADIOISOTOPES; Biological and medical sciences; Blood and lymphatic vessels; BLOOD PRESSURE; BLOOD VESSELS; BODY; CARBOXYLIC ACIDS; Cardiology. Vascular system; CARDIOVASCULAR DISEASES; CARDIOVASCULAR SYSTEM; CELL CONSTITUENTS; DAYS LIVING RADIOISOTOPES; DISEASES; Drug Carriers; ELECTRON CAPTURE RADIOISOTOPES; Experimental diseases; HYPERTENSION; Hypertension - metabolism; Hypertension - pathology; INDIUM 111; INDIUM ISOTOPES; Indium Radioisotopes; INTERMEDIATE MASS NUCLEI; ISOMERIC TRANSITION ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LIPOSOMES; Liposomes - metabolism; Male; MAMMALS; Medical sciences; MINUTES LIVING RADIOISOTOPES; MONOCARBOXYLIC ACIDS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANOIDS; ORGANS; PATHOGENESIS; RABBITS; RADIOISOTOPES; SYMPTOMS; TRACER TECHNIQUES; VASCULAR DISEASES; VERTEBRATES 550901 > Pathology > Tracer Techniques; Hypertension; Radiolabelling; Rabbit; Liposome; Cardiovascular disease; Lagomorpha; Pathology; Vertebrata; Experimental disease; Mammalia; Ligature; Animal; Drug targeting; Aorta; Vascular wall
• ISSN: 0194-911X; 1524-4563
• DOI: 10.1161/01.HYP.15.6.600

Using five sham-operated and seven aortic coarctation-induced hypertensive New Zealand White rabbits intravenously injected with neutral small unilamellar vesicles loaded with [“Inlnitrilotriacetic acid, we demonstrated in vivo that the normal aortic arterial wall participates in liposome uptake and that this uptake is increased in the hypertensive aortic wall by approximately threefold (p ≤ 0.0001). Among the three regions examined, aortic arch, thoracic aorta, and lower abdominal aorta, the difference in uptake between the normotensive and hypertensive arterial walls was significantly different, p ≤ 0.05, p ≤ 0.0001, and p ≤ 0.05, respectively. The uptake by the different regions of the hypertensive arterial wall is consistent with the pathological changes present in these areas. Furthermore, the extent of liposome uptake by the aortic wall is strongly correlated with the height of the blood pressure (r=0.85, p=0.001, n = 11). We conclude that neutral small unilamellar liposomes can be used to carry agents into the arterial wall in vivo in the study of hypertensive vascular disease and could be especially useful for the delivery of pharmacologically or biologically active agents that would otherwise be inactivated within the circulation or are impermeable to the arterial wall.