Effective treatment of mouse metastatic prostate cancer by low electric field enhanced chemotherapy

• Published in: The Prostate Vol. 66; no. 15; pp. 1620 - 1630
• Main Authors: Plotnikov, Alexander, Niego, Be'eri, Ophir, Rachel, Korenstein, Rafi, Keisari, Yona
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.11.2006; Wiley-Liss
• Subjects: Adenocarcinoma - drug therapy; Adenocarcinoma - mortality; Adenocarcinoma - secondary; Animals; Antibiotics, Antineoplastic - pharmacokinetics; Antibiotics, Antineoplastic - therapeutic use; Biological and medical sciences; Cell Line, Tumor; chemotherapy; Disease Models, Animal; Doxorubicin - pharmacokinetics; Doxorubicin - therapeutic use; Drug Screening Assays, Antitumor; Electrochemotherapy; Electroporation; electrostimulation; Gynecology. Andrology. Obstetrics; immunostimulation; low electric field; Male; Male genital diseases; Medical sciences; Mice; Mice, Inbred C57BL; Neoplasm Transplantation; Nephrology. Urinary tract diseases; prostate cancer; Prostatic Neoplasms - drug therapy; Prostatic Neoplasms - mortality; Prostatic Neoplasms - pathology; Spleen - drug effects; Survival Rate; Treatment Outcome; Tumors; Tumors of the urinary system; Urinary tract. Prostate gland; Andrology; Nephrology; Prostate disease; low electric field; electrostimulation; Metastasis; Efficiency; Advanced stage; Male genital diseases; Urinary system disease; Immunostimulation; Gynecology; Electrical stimulus; Rodentia; Malignant tumor; Vertebrata; Chemotherapy; Mammalia; Electric field; Treatment; Mouse; Low; Animal; Metastatic; Prostate cancer
• ISSN: 0270-4137; 1097-0045
• DOI: 10.1002/pros.20435

BACKGROUND We developed a new anti‐cancer treatment, which is a combination of chemotherapeutic agents and low electric field. In the present study we investigated its efficacy against prostate metastatic transgenic adenocarcinoma of mice (TRAMP). METHODS Mice with 5, 10, and 13 mm in diameter intracutaneous tumors received Low Electric Field Cancer Treatment‐Enhanced Chemotherapy (LEFCT‐EC) with doxorubicin (10 mg/kg), and monitored for survival, and primary and metastatic tumors growth. RESULTS The electric field increased the uptake of doxorubicin by TRAMP cells in vitro. In vivo use of LEFCT‐EC reduced tumor size, prolonged survival, and cured 36–93% of the animals, dependent on treated tumor size. LEFCT‐EC was more effective than surgery with or without chemotherapy. Part of the cured animals developed anti‐tumor immunity and immunosuppression, significantly decreased the effectiveness of the treatment. CONCLUSION Our results suggest that LEFCT‐EC is an effective method for the destruction of metastatic prostate tumors. Prostate 66: 1620–1630, 2006. © 2006 Wiley‐Liss, Inc.