Simplified Approaches for the Production of Monocyte-Derived Dendritic Cells and Study of Antigen Presentation in Bovine

• Published in: Frontiers in veterinary science Vol. 9; p. 891893
• Main Authors: Cunha, Patricia, Gilbert, Florence B., Bodin, Jennifer, Godry, Lise, Germon, Pierre, Holbert, Sebastien, Martins, Rodrigo Prado
• Format: Journal Article
• Language: English
• Published: Frontiers Media 09.06.2022; Frontiers Media S.A
• Subjects: antigen presentation; bovine; cell culture; dendritic cells; Immunology; Life Sciences; serum-free media; Veterinary Science; bovine; serum-free media; dendritic cells; antigen presentation; cell culture
• ISSN: 2297-1769; 2297-1769
• DOI: 10.3389/fvets.2022.891893

Dendritic cells are sentinels of the immune system responsible for the initiation of adaptive immune mechanisms. In that respect, the study of these cells is essential for a full understanding of host response to infectious agents and vaccines. In ruminants, the large blood volume facilitates the isolation of abundant monocytes and their derivation to other antigen-presenting cells such as dendritic cells and macrophages. However, the available protocols for the production of bovine monocyte-derived dendritic cells (moDCs) rely mostly on time-consuming and costly techniques such as density gradient centrifugation and magnetic sorting of cells. In this study, we describe a simplified protocol for the production of bovine moDC using conventional and serum-free media. We also employ moDC produced by this approach to carry out a flow cytometry-based antigen presentation assay adapted to blood fresh or frozen cells. The experimental strategies described here might enable the setup of studies involving a large number of individuals, requiring a large number of dendritic cells, or relying on the utilization of cryopreserved blood cells. These simplified protocols might contribute to the elucidation of cell-mediated immune responses in bovine.