Phage selection assisted by Sfp phosphopantetheinyl transferase-catalyzed site-specific protein labeling

Phosphopantetheinyl transferases (PPTase) Sfp and AcpS catalyze a highly efficient reaction that conjugates chemical probes of diverse structures to proteins. PPTases have been widely used for site-specific protein labeling and live cell imaging of the target proteins. Here we describe the use of PP...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1266; p. 161
Main Authors Zhao, Bo, Zhang, Keya, Bhuripanyo, Karan, Wang, Yiyang, Zhou, Han, Zhang, Mengnan, Yin, Jun
Format Journal Article
LanguageEnglish
Published United States 2015
Subjects
Bacterial Proteins - chemistry
Biotin - chemistry
Biotinylation
Escherichia coli
Peptide Library
Protein Engineering
Staining and Labeling
Streptavidin - chemistry
Transferases (Other Substituted Phosphate Groups) - chemistry
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Summary:Phosphopantetheinyl transferases (PPTase) Sfp and AcpS catalyze a highly efficient reaction that conjugates chemical probes of diverse structures to proteins. PPTases have been widely used for site-specific protein labeling and live cell imaging of the target proteins. Here we describe the use of PPTase-catalyzed protein labeling in protein engineering by facilitating high-throughput phage selection.
ISSN:1940-6029
DOI:10.1007/978-1-4939-2272-7_11