Microencapsulated Multifunctionalized Graphene Oxide Equipped with Chloroquine for Efficient and Sustained siRNA Delivery

• Published in: BioMed research international Vol. 2022; pp. 5866361 - 16
• Main Authors: Imani, Rana, Prakash, Satya, Vali, Hojatollah, Presley, John F., Faghihi, Shahab
• Format: Journal Article
• Language: English
• Published: United States Hindawi 2022; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Acids; Alginates; Alginic acid; Breast cancer; Cancer therapies; Cell culture; Chloroquine; Chloroquine - pharmacology; Conjugation; Cytoplasm; Diamines; Dosage and administration; Drug Carriers; Folic Acid; Gene therapy; Graphene; Graphite; Health aspects; Intracellular; Lysosomes; Microencapsulation; Microspheres; Nanoparticles; Nanotechnology; Patient outcomes; Polyethylene glycol; Polyethylene Glycols; Reagents; RNA, Small Interfering - genetics; siRNA; Spectrum analysis; Vitamin B; Iran; Canada; United States > US; Montreal Quebec Canada
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2022/5866361

A multifunctionalized graphene oxide (GO)-based carrier with conjugation of aminated-polyethylene glycol (PEG-diamine), octaarginine (R8), and folic acid (FA), which also contains chloroquine (CQ), a lysosomotropic agent, is introduced. The cellular uptake mechanisms and intracellular targeting of FA-functionalized nanocarriers are examined. The localized releases of CQ and siRNA intracellular delivery are evaluated. Microencapsulation of the nanocarrier complexed with genes in layer-by-layer coating of alginate microbeads is also investigated. The covalently coconjugated FA with PEG and R8 provides a stable formulation with increased cellular uptake compared to FA-free carrier. The CQ-equipped nanocarrier shows a 95% release of CQ at lysosomal pH. The localized release of the drug inside the lysosomes is verified which accelerates the cargo discharge into cytoplasm.