New insights on pestivirus infections in transhumant sheep and sympatric Pyrenean chamois (Rupicapra p. pyrenaica)

• Published in: Veterinary microbiology Vol. 217; pp. 82 - 89
• Main Authors: Colom-Cadena, Andreu, Espunyes, Johan, Cabezón, Oscar, Fernández-Aguilar, Xavier, Rosell, Rosa, Marco, Ignasi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2018; Elsevier BV
• Subjects: Agricultural economics; Alpine environments; Antibodies; Border disease; Border disease virus; Bovine viral diarrhea virus; Disease transmission; Economic impact; Enzyme-linked immunosorbent assay; Epidemiology; Infections; Livestock; Microbiology; Outbreaks; Ovine; Population studies; Pyrenees; Rupicapra pyrenaica; Serology; Sheep; Spleen; Strains (organisms); Sympatric populations; Transhumance; Viruses; Wildlife; Wildlife conservation; Border disease virus; Ovine; Bovine viral diarrhea virus; Pyrenees; Wildlife; Transhumance
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2018.03.003

•Transhumance favours BDV transmission by joining sheep feedlots in alpine meadows.•BDV transmission between sheep and Pyrenean chamois occurs occasionally.•Transhumant sheep and Pyrenean chamois mainly have independent pestivirus cycles.•BDV strains from Pyrenean chamois and sheep are closely phylogenetically related.•BDV epidemiology in chamois populations may favour circumstantial contacts with sheep. Border Disease Virus (BDV) causes health and economic impact on livestock and is also of importance in wildlife conservation as it causes high mortality outbreaks in Pyrenean chamois (Rupicapra pyrenaica pyrenaica). Pastoral practices are known as a main interspecies pathogen transmission. Hence, the presence of pestivirus in transhumant sheep flocks and sympatric chamois was assessed in areas with different epidemiological scenarios of chamois BDV infections. Moreover, the present study had also the goal to identify if inter-specific infections occurred and when they happened. Five sheep flocks grazing in two alpine areas in the Pyrenees with two different BDV epidemiological scenarios in chamois populations were studied during two transhumant seasons. Sheep were sampled before and after transhumance. Pyrenean chamois sera and spleen samples from both areas where also studied during the same period. Antibodies against BDV were assessed by means of ELISA and VNT. A qRT-PCR was used in order to detect the virus. Seroprevalence in sheep ranged between 0 and 91.1% at the flock level. Chamois were found to have high seroprevalences (52.9–77.7%) in both areas, and four new BDV isolates were sequenced. One sheep farm presented persistent BDV circulation and three showed low BDV circulation. The after-transhumance period was identified as the moment when viral transmission occured in the first farm, associated to BDV strains of domestic origin, according to VNT results. However, the BDV isolate was genetical closely related to previous BDV strains from chamois origin. In another farm, antibodies in two of the three positive sera were associated to infection with a chamois-like BDV strain. Altogether indicates that occasional viral transmission from chamois to sheep may occur.