Metabolite mapping of human filarial parasite, Brugia Malayi with nuclear magnetic resonance

• Published in: Magnetic resonance imaging Vol. 17; no. 10; pp. 1503 - 1509
• Main Authors: Shukla–Dave, Amita, Degaonkar, M, Roy, Raja, Murthy, P.K, Murthy, P.S.R, Raghunathan, P, Chatterjee, R.K
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.12.1999; Elsevier Science
• Subjects: 1H Magnetic Resonance Imaging (MRI); 31P Nuclear Magnetic Resonance (NMR) spectroscopy; Animals; Biological and medical sciences; Brugia malayi; Brugia malayi - anatomy & histology; Brugia malayi - isolation & purification; Brugia malayi - metabolism; Carbohydrate metabolism; Diseases caused by nematodes; Female; Filariases; Filariasis - diagnosis; Filariasis - metabolism; Helminthic diseases; Infectious diseases; Lipid metabolism and filariasis; Lymphatic filariases; Magnetic Resonance Imaging; Magnetic Resonance Spectroscopy; Male; Mastomys coucha; Medical sciences; Parasitic diseases; Phospholipids - metabolism; Rats; Sugar Phosphates - metabolism; Testicular Diseases - diagnosis; Testicular Diseases - metabolism; Testicular Diseases - parasitology; Testis - metabolism; Testis - pathology; 31P Nuclear Magnetic Resonance (NMR) spectroscopy; Carbohydrate metabolism; Lipid metabolism and filariasis; Brugia malayi; Mastomys coucha; 1H Magnetic Resonance Imaging (MRI); Rat; Metabolite; Rodentia; Parasite; Nuclear magnetic resonance imaging; Spectral analysis; In vivo; Vertebrata; Spectrometry; Mammalia; Ex vivo; Animal; Helmintha; Medical imagery; Nemathelminthia; Nuclear magnetic resonance; Invertebrata; Nematoda
• ISSN: 0730-725X; 1873-5894
• DOI: 10.1016/S0730-725X(99)00091-0

Metabolite mapping of human filarial parasite, Brugia malayi was carried out in vitro as well as in situ in host Mastomys coucha by 31P nuclear magnetic resonance (NMR) spectroscopy. Detection of parasites by visualizing contrast spots due to pathologic changes was observed by 1H magnetic resonance imaging (MRI). Major metabolites of adult B. malayi observed by 31P-NMR spectroscopy were of sugar phosphates (SP), phosphomonoesters (PME), glycerophosphoryl-ethanolamine (GPE), -choline (GPC), phosphoenolpyruvate (PEP), inorganic phosphate (Pi), nucleoside diphosphosugar and nucleotides-mono, -di and -tri phosphates. PEP and GPC were present in high concentration; PEP being the major energy reservoir and GPC the major phospholipid in this species of filaria. The 31P NMR spectra of testis of mastomys, showed seven major peaks of SP, PME, phosphocreatine (PCr), phosphodiesters (PDE), Pi, and nucleotides di- and tri-phosphates. The 31P-NMR spectra of testis of B. malayi infected animal also consisted of seven major peaks with significant decrease in the SP and PME peak showing changes in the carbohydrate and lipid metabolism of filaria infected testis. Thus, in vivo 31P MRS provided a non-invasive assessment of tissue bioenergetics and phospholipid metabolism.