Identification of the adrenoceptor subtypes expressed on GABAergic neurons in the anterior hypothalamic area and rostral zona incerta of GAD65-eGFP transgenic mice

• Published in: Neuroscience letters Vol. 422; no. 3; pp. 153 - 157
• Main Authors: Shin, Seung Yub, Yang, Jian Hua, Lee, Hang, Erdélyi, Ferenc, Szabó, Gábor, Lee, So Yeong, Ryu, Pan Dong
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 18.07.2007; Elsevier
• Subjects: Animals; Biological and medical sciences; Female; Fundamental and applied biological sciences. Psychology; gamma-Aminobutyric Acid - metabolism; Glutamate Decarboxylase - genetics; Glutamate Decarboxylase - metabolism; Green Fluorescent Proteins; Immunohistochemistry; Isoenzymes - genetics; Isoenzymes - metabolism; Male; Mice; Mice, Transgenic; Neurons - metabolism; Paraventricular Hypothalamic Nucleus - metabolism; Peri-PVN area; Receptors, Adrenergic - biosynthesis; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; Vertebrates: nervous system and sense organs; α 1A; α 1B; α 2A-Adrenoceptor; Peri-PVN area; α 1A; α 1B; α 2A-Adrenoceptor; Enzyme; Zona incerta; Rodentia; Central nervous system; Transgenic animal; Lyases; Glutamate decarboxylase; Hypothalamus; α1A; Encephalon; α1B; Carbon-carbon lyases; Vertebrata; Mammalia; Mouse; α2A-adrenergic receptor; Carboxy-lyases; α2A-Adrenoceptor; Adrenergic receptor; Gabaergic neuron
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2007.05.060

GABA is a major neurotransmitter in the hypothalamus. In particular, neurons in the paraventricular nucleus (PVN) of the hypothalamus receive dense GABAergic inputs from peri-PVN regions. The noradrenergic system has been reported as a modulator of GABAergic transmission to the PVN. Previous electrophysiological and morphological studies support the presence of adrenoceptors on GABAergic neurons innervating the PVN. In this study, we identified three adrenoceptors on GABAergic neurons in the peri-PVN region, focusing on the anterior hypothalamic area (AHA) and rostral zona incerta (ZIr). GABAergic neurons were identified using enhanced green fluorescent protein (eGFP), followed by single cell RT-PCR analysis of the GABA synthetic enzymes, glutamic acid decarboxylase (GAD)65 and/or GAD67. Single cell RT-PCR data revealed the expression of α 1A-, α 1B- and α 2A-adrenoceptor mRNA on GABAergic neurons in AHA and ZIr. Additionally, immunohistochemical studies showed that the immunoreactivities of α 1A-, α 1B- and α 2A-adrenoceptor were colocalized with eGFP-expressing neurons in AHA and ZIr. The present findings suggest the contribution of adrenoceptors to the modulation of GABAergic neurons in AHA and ZIr.