Determination of 2,3-benzodiazepine derivatives in rat plasma by high-performance liquid chromatography

• Published in: Journal of Chromatography A Vol. 846; no. 1; pp. 165 - 168
• Main Authors: Rizzo, M, De Sarro, G, Gitto, R, Zappalà, M, Chimirri, A
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 18.06.1999; Elsevier
• Subjects: Analysis; Animals; Benzodiazepines; Benzodiazepines - blood; Benzodiazepines - pharmacokinetics; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Excitatory Amino Acid Antagonists - blood; Excitatory Amino Acid Antagonists - pharmacokinetics; General pharmacology; Medical sciences; Pharmacology. Drug treatments; Rats; Rats, Sprague-Dawley; Reference Standards; Reproducibility of Results; Sensitivity and Specificity; Spectrophotometry, Ultraviolet; Benzodiazepines; Biological fluid; Intraperitoneal administration; Chemical analysis; Rat; Nitrogen heterocycle; Metabolite; Rodentia; HPLC chromatography; Anticonvulsant; Glutamate receptor; Blood; Blood plasma; Kainate receptor; Vertebrata; AMPA receptor; Mammalia; Ultraviolet detector; Animal; Bicyclic compound; Aromatic compound; Antagonist; Pharmacokinetics; Quantitative analysis
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(99)00011-4

A simple high-performance liquid chromatographic method with ultraviolet detection at 240 nm for determination of a novel AMPA/kainate antagonist 1-(4′-aminophenyl)-3,5-dihydro-7,8-dimethoxy-2,3-benzodiazepine (2,3-BZ 6), and its derivatives in rat plasma is described. The procedure involves a fast extraction of the drugs from the plasma spiked with an internal standard. The samples are applied to a pre-packed glass column and drugs are eluted using ethyl acetate. A linear response was observed over the examined concentration range. The lower limit of detection of 2,3-BZ 6 was 5.5 ng/ml. The assay has been used to determine the time course of plasma levels of the 2,3-benzodiazepine derivatives in Sprague–Dawley rats.