Upregulation of Phagocytic Clearance of Apoptotic Cells by Autoimmune Regulator

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 30; no. 2; pp. 145 - 148
• Main Author: 石亮 胡丽华 李一荣
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.04.2010; Department of Clinical Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
• Subjects: AIRE Protein; Apoptosis; Autoimmunity - immunology; DNA, Complementary - genetics; Epithelial Cells - cytology; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Genetic Vectors; Granulocytes - cytology; HL-60 Cells; Humans; Medicine; Medicine & Public Health; Phagocytes - physiology; Phagocytosis; rac1 GTP-Binding Protein - genetics; rac1 GTP-Binding Protein - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Transcription Factors - metabolism; Transfection; Up-Regulation; Western印迹; 免疫调节; 全长cDNA; 凋亡细胞; 吞噬功能; 清除作用; 细胞转染; 重组表达载体; phagocytosis; autoimmune regulator; apoptosis; rac1 protein; racl protein
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-010-0202-5

To investigate the effect of autoimmune regulator （AIRE） on phagocytic clearance of apoptotic cells, a recombinant expression vector containing full-length human AIRE cDNA was transfected into 16HBE cells. After incubation with transfected 16HBE cells, engulfment of apoptotic HL-60 cells induced by camptothecin was detected by myeloperoxidase （MPO） staining. The change in the expression of Rac 1 in transfected 16HBE cells was determined by RT-PCR and Western blotting. The results showed that the phagocytosis percentage of the experimental group, the mock trans- fection group and the negative control group （non-apoptotic cells） was （25.50±3.67）%, （6.25±1.58）% and （1.0±0.67）%, respectively. Moreover, the expressions of Rac 1 mRNA and protein were up-regulated in AIRE-transfected 16HBE cells, suggesting that AIRE may function as a regulator in the phagocytic clearance of apoptotic cells by promoting the expression of Rac 1.