Simultaneous solid-phase extraction combined with liquid chromatography with ultraviolet absorbance detection for the determination of remifentanil and its metabolite in dog plasma

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 783; no. 1; pp. 103 - 111
• Main Authors: Kabbaj, M, Varin, F
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05.01.2003
• Subjects: Analgesics, Opioid - blood; Analgesics, Opioid - pharmacokinetics; Animals; Chromatography, High Pressure Liquid - methods; Dogs; Piperidines - blood; Piperidines - pharmacokinetics; Remifentanil; Reproducibility of Results; Sensitivity and Specificity; Spectrophotometry, Ultraviolet - methods; Remifentanil
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/S1570-0232(02)00524-X

To establish pharmacokinetic/pharmacodynamic relationships, a selective and specific high-performance liquid chromatographic method was developed for the quantitation of remifentanil and its metabolite in dog plasma. The assay involves a solid-phase extraction and a reversed-phase chromatographic separation with ultraviolet detection ( λ=210 nm). The calibration curves are linear in the range of 7.89–1500 ng ml −1. Intra-day assay variability is less than 7% for all standards evaluated. Good recovery, linearity, accuracy, and precision were achieved with the assay that proved readily applicable to pharmacokinetic studies in dogs.