Changes in the activity of the multifunctional β-glycosyl hydrolase (Cel44C-Man26A) from Paenibacillus polymyxa by removal of the C-terminal region to minimum size

• Published in: Biotechnology letters Vol. 30; no. 6; pp. 1061 - 1068
• Main Authors: Cho, Kye-Man, Math, Renukaradhya K, Hong, Su-Young, Asraful Islam, Shah Md, Kim, Jong-Ok, Hong, Sun-Joo, Kim, Hoon, Yun, Han-Dae
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.06.2008; Springer Netherlands
• Subjects: active sites; Amino Acid Sequence; amino acids; Applied Microbiology; Artificial Gene Fusion; Bacteria - enzymology; Bacteria - genetics; Bacteria - metabolism; Bacterial Proteins - biosynthesis; Bacterial Proteins - metabolism; beta-mannosidase; beta-Mannosidase - metabolism; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cellulases - metabolism; Cloning, Molecular; Codon, Nonsense - genetics; DNA, Recombinant - biosynthesis; Electrophoresis, Polyacrylamide Gel; Endo-1,4-beta Xylanases - metabolism; endo-1,4-beta-glucanase; enzyme activity; fibronectins; Glycoside Hydrolases - biosynthesis; Glycoside Hydrolases - metabolism; Hydrogen-Ion Concentration; licheninase; Life Sciences; Microbiology; Molecular Sequence Data; Mutagenesis, Insertional; Original Research Paper; Paenibacillus polymyxa; xylanases; 26A gene; 44C; Multifunctional enzyme; GS01; Truncated Cel44C-Man26A proteins
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-008-9640-6

Paenibacillus polymyxa GS01 secretes Cel44C-Man26A as a multifunctional enzyme with cellulase, xylanase, lichenase, and mannanase activities. Cel44C-Man26A consists of 1,352 amino acids in which present a catalytic domain (CD) of the glycosyl hydrolase family 44 (GH44), fibronectin domain type 3 (Fn3), catalytic domain of glycosyl hydrolase family 26 (GH26), and a cellulose-binding module type 3 (CBM3). A truncated Cel44C-Man26A protein, consisting of 549 amino acid residues, reacted as a multifunctional mature enzyme despite the absence of the 10 amino acids containing GH44, Fn3, GH26, and CBM3. However, the multifunctional activity was not found in the mature Cel44C-Man26A protein truncated to less than 548 amino acids. The truncated Cel44C-Man26A proteins showed the optimum pH for the lichenase activity was pH 7.0, pH 6.0 for the xylanase and mannanase, and pH 5.0 for the cellulase. The truncated Cel44C-Man26A proteins exhibited enzymatic activity 40-120% higher than the full-length Cel44C.