A reverse (antibody capture) enzyme-linked immunosorbent assay for detection of antisperm antibodies in sera and genital tract secretions

A reverse (antibody capture) enzyme-linked immunosorbent assay (ELISA) for detection of antisperm antibodies has been developed. The assay enables detection of immunoglobulin (Ig)M, IgG, IgA, or IgM, IgG, and IgA—antisperm antibodies in serum, cervical mucus, and seminal plasma samples. The reverse...

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Bibliographic Details
Published inFertility and sterility Vol. 54; no. 5; pp. 894 - 901
Main Authors Shai, Shafrira, Bar-Yoseph, Naomi, Peer, Eitan, Naot, Yehudith
Format Journal Article
LanguageEnglish
Published New York, NY Elsevier Inc 01.11.1990
Elsevier Science
Subjects
Adult
Antibodies - analysis
Antibodies - blood
Antibodies - immunology
Biological and medical sciences
Birth control
Bromelains - pharmacology
Cervix Mucus - chemistry
Enzyme-Linked Immunosorbent Assay - methods
Female
Gynecology. Andrology. Obstetrics
Humans
Immunoglobulin A - analysis
Immunoglobulin A - immunology
Immunoglobulin G - analysis
Immunoglobulin G - immunology
Immunoglobulin M - analysis
Immunoglobulin M - immunology
Infertility - immunology
Male
Medical sciences
Semen - chemistry
Spermatozoa - immunology
Sterility. Assisted procreation
Human
Screening
Spermatozoa
Antibody
Semen
Cervical mucus
Diagnosis
Sterility
ELISA assay
Blood plasma
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Summary:A reverse (antibody capture) enzyme-linked immunosorbent assay (ELISA) for detection of antisperm antibodies has been developed. The assay enables detection of immunoglobulin (Ig)M, IgG, IgA, or IgM, IgG, and IgA—antisperm antibodies in serum, cervical mucus, and seminal plasma samples. The reverse ELISA is more specific and sensitive than conventional ELISA in detecting human antisperm antibodies of different isotypes. Using this assay, statistically significant differences in levels of antibodies between infertile and fertile individuals were demonstrated in sera and in genital tract secretions. Studies with 143 infertile couples revealed that the presence of antibodies in sera was not necessarily reflected in individual’s genital tract secretion and vice versa. These data emphasize the importance of detecting antisperm antibodies in sera as well as in genital tract secretions for correct evaluation of sperm immunity.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0015-0282
1556-5653
DOI:10.1016/S0015-0282(16)53952-1