Oscillatory electroosmosis-enhanced intra/inter-particle liquid transport and its primary applications in the preparative electrochromatography of proteins

• Published in: Journal of Chromatography A Vol. 921; no. 1; pp. 93 - 98
• Main Authors: Liu, Zheng, Yin, Gang, Feng, Shaohua, Wang, Donghai, Ding, Fuxin, Yuan, Naiju
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 29.06.2001; Elsevier
• Subjects: Adsorption; Albumin; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chromatography, Micellar Electrokinetic Capillary - methods; Fundamental and applied biological sciences. Psychology; Holoproteins; Osmosis; Other proteins; Proteins; Proteins - isolation & purification; Proteins; Albumin; Affinity electrochromatography; Electroosmosis; Preparative chromatography; Electrochromatography; Human; Purification; Bovine; Adsorption isotherm; Serum albumin; Serum protein; Operating conditions; Separation method; Vertebrata; Mammalia; Affinity chromatography; Animal; Artiodactyla; Electric field effect; Ungulata; Breakthrough curve
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(01)00746-4

The concept of generating an oscillatory electroosmotic flux inside the porous particle to enhance the intra-particle mass transport was presented and a new kind of electrochromatography carried out in a five-compartment electrolyzer were developed. The adsorbent was packed in the central compartment, while the neighboring compartments were used as the elution compartments and the electrode compartments, respectively. Chromatographic separations of human serum albumin on Blue Sepharose Fast Flow, bovine serum albumin (BSA) on DEAE–Sepharose Fast Flow, and BSA on hydroxyapatite were carried out, respectively. The adsorption isotherms were shown to be independent of electric field, while the increase in the electric field strength resulted in a linear increase in the magnitude of electroosmotic flux and the improvement of the breakthrough behavior in all cases. The experiment results have demonstrated the effectiveness of the oscillatory electroosmosis in enhancing intra- and inter-particle mass transport and its high potential to large-scale chromatography.