Dopaminergic neuromodulation of synaptic transmission between mitral and granule cells in the teleost olfactory bulb

• Published in: Journal of neurophysiology Vol. 107; no. 5; pp. 1313 - 1324
• Main Authors: Kawai, Takafumi, Abe, Hideki, Oka, Yoshitaka
• Format: Journal Article
• Language: English
• Published: United States 01.03.2012
• Subjects: Animals; Carassius auratus; Dopamine - physiology; Dopaminergic Neurons - physiology; Freshwater; Goldfish; Nerve Net - physiology; Neurotransmitter Agents - physiology; Olfactory Bulb - cytology; Olfactory Bulb - physiology; Synaptic Transmission - physiology; Teleostei
• ISSN: 0022-3077; 1522-1598
• DOI: 10.1152/jn.00536.2011

A growing body of evidence suggests that teleosts are important models for the study of neural processing of olfactory information, and the functional role of dopamine (DA), which is a potent neuromodulator endogenous to the mammalian olfactory bulb, has been one of the strongest focuses in this field. However, the cellular mechanisms of dopaminergic neuromodulation in olfactory bulbar neural circuits have not been fully understood. We investigated such mechanisms by using the goldfish, which offers several advantages for analyzing olfactory information processing by electrophysiological methods. First, we found in the olfactory bulb that numerous cell bodies of the dopaminergic neurons are mainly distributed in the mitral cell layer and extend fine processes to the glomerular layer. Next, we made in vitro field potential recordings and showed that synaptic transmissions from mitral to granule cells were suppressed by DA application. DA also increased the paired-pulse ratio, suggesting that the suppression of synaptic transmission is caused by a decrease in presynaptic glutamate release from the mitral cells. Furthermore, DA significantly suppressed the oscillatory activity of the olfactory bulb in response to olfactory stimuli. Although DA suppresses the synaptic inputs from the olfactory nerve to the olfactory bulbar neurons in mammals, this phenomenon was not observed in the goldfish. These findings indicate that suppression of the mitral to granule cell synaptic transmission in the reciprocal synapses plays an important role in the negative regulation of olfactory responsiveness in the goldfish olfactory bulb.