Competitive immunoassay for staphylococcal enterotoxin A using capillary electrophoresis with laser-induced fluorescence detection

Staphylococcal enterotoxins are a family of toxic proteins secreted by S. aureus. Using capillary electrophoresis (CE) linked with laser-induced fluorescence, a highly sensitive and selective assay using antibody–antigen recognition was developed for the determination of Staphylococcal enterotoxin A...

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Bibliographic Details
Published inJournal of Chromatography A Vol. 853; no. 1-2; pp. 545 - 553
Main Authors Lam, Michael T, Wan, Q.H, Boulet, Camille A, Le, X.Chris
Format Journal Article Conference Proceeding
LanguageEnglish
Published Amsterdam Elsevier B.V 20.08.1999
Elsevier
Subjects
Bacteriology
Biological and medical sciences
Electrophoresis, Capillary - methods
Enterotoxins
Enterotoxins - analysis
Fluorescence
Fundamental and applied biological sciences. Psychology
Immunoassay - methods
Lasers
Microbiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Proteins
Staphylococcus aureus - chemistry
Toxins
Proteins
Immunoassay
Laser-induced fluorescence
Toxins
Enterotoxins
Fluorescence detector
Zone electrophoresis
Capillary electrophoresis
Laser induced fluorescence
Antigen antibody reaction
Immunological method
Toxin
Analysis method
Fluorescent labelling
Fluorescein
Enterotoxin
Bacteria
Micrococcales
Micrococcaceae
Staphylococcus aureus
Quantitative analysis
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Summary:Staphylococcal enterotoxins are a family of toxic proteins secreted by S. aureus. Using capillary electrophoresis (CE) linked with laser-induced fluorescence, a highly sensitive and selective assay using antibody–antigen recognition was developed for the determination of Staphylococcal enterotoxin A. Staphylococcal enterotoxin A (SEA) was chemically labeled with fluorescein and the product was used as a fluorescent tracer. A competitive assay was developed to detect SEA at concentrations between 0.3 nM and 6.5 nM with standard deviations of less than 5%. The detection limit was found to be 3 amol with the potential improvement by further optimization of the assay. No cross-reactivity between staphylococcal enterotoxin B and the SEA antibody was found at the concentrations used for the CE immunoassay.
ISSN:0021-9673
DOI:10.1016/S0021-9673(99)00677-9