Short-term oral oleoyl-estrone decreases the expression of ghrelin in the rat stomach

• Published in: Regulatory peptides Vol. 152; no. 1; pp. 79 - 81
• Main Authors: Ferrer-Lorente, Raquel, Fernández-López, José-Antonio, Alemany, Marià, Cabot, Cristina
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 08.01.2009; Elsevier
• Subjects: Animals; Anti-Obesity Agents - administration & dosage; Biological and medical sciences; Cholecystokinin - genetics; Cholecystokinin - metabolism; Eating - physiology; Estrone - administration & dosage; Estrone - analogs & derivatives; Food intake; Fundamental and applied biological sciences. Psychology; Gene Expression; Ghrelin - genetics; Ghrelin - metabolism; Glucagon-Like Peptide 1 - genetics; Glucagon-Like Peptide 1 - metabolism; Intestinal peptides; Male; Oleic Acids - administration & dosage; Oleoyl-estrone; Peptide YY - genetics; Peptide YY - metabolism; Rats; Rats, Wistar; Short-term satiety; Stomach - drug effects; Stomach - metabolism; Vertebrates: endocrinology; Intestinal peptides; Short-term satiety; Oleoyl-estrone; Food intake; Short term; Stomach; Peptides; Rat; Digestive system; Rodentia; Gut; Feeding; Vertebrata; Mammalia; Ghrelin; Animal; Satiety; Estrone
• ISSN: 0167-0115; 1873-1686
• DOI: 10.1016/j.regpep.2008.09.004

Oleoyl-estrone (OE) mobilizes body fat and decreases food intake. The precise mechanism of its modulation of appetite is unknown. Since the effects of OE on food intake appear early, here we studied the effect of OE on the expression of gut peptides that affect short-term ingestive behavior: ghrelin, leptin, CCK, PYY, and GLP-1. Two hours after a single OE dose, adult male rats were killed and their stomach fundus and intestine sections were dissected and processed for real-time PCR amplification. Semi-quantitative estimation of gene mRNA tissue levels showed that OE markedly decreased ghrelin expression in the stomach; leptin mRNA was unchanged; CCK mRNA decreased in the proximal intestine while PYY and GLP-1 expression in the intestine was not altered. Our results indicate that the short-term decrease in food intake induced by OE may be essentially the consequence of a marked decrease in the expression of ghrelin in the stomach.