Voltammetric methods for analytical determination of fleroxacin in Quinodis® tablets

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 22; no. 6; pp. 925 - 932
• Main Authors: KAPETANOVIC, V, MILOVANOVIC, L, ALEKSIC, M, IGNJATOVIC, L
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.07.2000; Elsevier Science
• Subjects: Adsorptive stripping voltammetry; Analysis; Anti-Infective Agents - analysis; Biological and medical sciences; Calibration; Determination; dpp Polarography; Electrochemistry; Electrodes; Fleroxacin; Fleroxacin - analysis; General pharmacology; Hydrogen-Ion Concentration; Indicators and Reagents; Medical sciences; Pharmacology. Drug treatments; Polarography; Tablets; Fleroxacin; dpp Polarography; Adsorptive stripping voltammetry; Determination; Tablets; Fluoroquinolone derivatives; Adsorption; Polarography; Electrochemistry; Dosage form; Tablet; Antibacterial agent; Quinolone derivatives; Cathodic stripping voltammetry; Quantitative analysis
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/S0731-7085(00)00289-2

The direct current (dc) and differential pulse (dp) polarographic reduction of fleroxacin was done in a wide pH range from 2.48 to 13.00. The appropriate buffer choice was made for its dp polarographic determination in a range from 1.845 to 16.926 μg/ml, at pH 8.50. The adsorptive properties of fleroxacin were investigated in order to achieve an increase in sensitivity and a possibility of fleroxacin determination by applying the adsorptive stripping voltammetric method. The adsorptive processes at the hanging mercury drop electrode were investigated in Britton–Robinson and borate buffers. Adsorptive preconcentration followed by differential-pulse cathodic stripping showed one wave at ∼−1.1 V being the most sensitive for analytical determination of fleroxacin. Two linear ranges were obtained, the first one from 18.465 to 258.51 ng/ml, and the second one from 3.693 to 18.465 ng/ml.