Prohormone convertase-1 is essential for conversion of chromogranin A to pancreastatin
The purpose of this study was to test the hypothesis that the endoprotease, prohormone convertase-1 (PC-1), is involved in the processing of the precursor protein chromogranin A (CGA) to a smaller peptide called pancreastatin (PST). A human pancreatic carcinoid cell line (BON) that expresses PC-1, C...
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Published in | Regulatory peptides Vol. 83; no. 2; pp. 123 - 127 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Shannon
Elsevier B.V
15.09.1999
Amsterdam Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | The purpose of this study was to test the hypothesis that the endoprotease, prohormone convertase-1 (PC-1), is involved in the processing of the precursor protein chromogranin A (CGA) to a smaller peptide called pancreastatin (PST). A human pancreatic carcinoid cell line (BON) that expresses PC-1, CGA and PST was stably transfected with antisense PC-1 mRNA. BON cells expressing antisense PC-1 mRNA showed nearly complete abolishment of PC-1 protein (∼95% reduction) and an 80% reduction in cell content of PST immunoreactivity (PST-IR) as assessed by high-performance liquid chromatography in combination with measurement of PST-IR. These findings indicate that PC-1 is essential for processing CGA to PST. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0167-0115 1873-1686 |
DOI: | 10.1016/S0167-0115(99)00061-0 |