Extraction and Isolation of Two Polysaccharides from Chloranthus japonicus Sieb. and Evaluation of Their Anti-Gastric Cancer Activities

• Published in: Molecules (Basel, Switzerland) Vol. 29; no. 9; p. 2043
• Main Authors: Liu, Ju, Li, Wenfeng, Jin, Lu, Wang, Yingchao, Xu, Xinjun, Ma, Enyao, Yang, Depo, Zhao, Zhimin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 29.04.2024
• Subjects: Acids; anti-gastric cancer; Antineoplastic Agents, Phytogenic - chemistry; Antineoplastic Agents, Phytogenic - isolation & purification; Antineoplastic Agents, Phytogenic - pharmacology; Caryophyllaceae - chemistry; Cell Line, Tumor; Cell Proliferation - drug effects; Chloranthus japonicus Sieb; Fourier transforms; Gastric cancer; Glucose; Humans; Molecular Weight; Plant Extracts - chemistry; Plant Extracts - pharmacology; polysaccharide; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Scanning electron microscopy; Spectrum analysis; Stomach Neoplasms - drug therapy; Stomach Neoplasms - pathology; structure features; China; Japan; anti-gastric cancer; polysaccharide; structure features; Chloranthus japonicus Sieb
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules29092043

Two unreported heteropolysaccharides, denoted as YCJP-1 and YCJP-2, were isolated from the herbs of . YCJP-1 was a heteropolysaccharide composed of glucose, galactose, arabinose, mannose, rhamnose, and a minor proportion of uronic acids, with the molecular weight mainly distributed in the 74,475-228,443 Da range. YCJP-2 was mainly composed of glucose, mannose, and galactose, with the molecular weights ranging from 848 to 5810 Da. To further evaluate the anti-gastric cancer effects of , the inhibitory effects of the crude polysaccharide (YCJP) and the purified polysaccharides (YCJP-1 and YCJP-2) were determined using a CCK-8 assay and colon-forming assay on MGC-803 and AGS gastric cancer cell lines. Our results showed that YCJP, YCJP-1, and YCJP-2 possess prominent inhibitory effects on the proliferation of MGC-803 and AGS cells, and the AGS cell was more sensitive to YCJP, YCJP-1, and YCJP-2. Moreover, YCJP-2 demonstrated superior anti-gastric cancer effects compared to YCJP-1. This could potentially be attributed to YCJP-2's higher glucose content and narrower molecular weight distribution.