Chiral determination of amino acids by capillary electrophoresis and laser-induced fluorescence at picomolar concentrations

• Published in: Journal of Chromatography A Vol. 894; no. 1; pp. 259 - 266
• Main Authors: Vandenabeele-Trambouze, O, Albert, M, Bayle, C, Couderc, F, Commeyras, A, Despois, D, Dobrijevic, M, Grenier Loustalot, M.-F
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 13.10.2000; Elsevier
• Subjects: Amino acids; Amino Acids - analysis; Aminoisobutyric acid; Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Electrophoresis, Capillary - methods; Exact sciences and technology; Fluorescence; Isovaline; Lasers; Other chromatographic methods; Sensitivity and Specificity; Spectrometry, Fluorescence; Stereoisomerism; Surfactants; Aminoisobutyric acid; Enantiomer separation; Isovaline; Amino acids; Diastereomer separation; Buffer composition; Surfactants; Trace analysis; Fluorescence detector; Separation; Phase composition; Capillary electrophoresis; Non ionic surfactant; Laser induced fluorescence; Derivatization; Experimental study; Mobile phase; Ethylene oxide polymer; α-Aminoacid; Ultraviolet detector; Optical resolution; Fluorescent labelling; Enantiomer
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(00)00702-0

In this publication we present results on the determination of enantiomers of amino acids at very low concentrations. A fluoresceine-based chiral dye was synthesized to allow the separation of diastereoisomers of d- and l-amino acids. We used capillary electrophoresis with different non-ionic surfactants (Brij). The separation parameters were optimized and separations of d- and l-isovaline, an unusual terrestrial amino acid, were obtained. The sensitivity limits were also determined using a commercial laser-induced fluorescence detector. The quantitation of these amino acids is very important to understand the process of chiral selection on Earth.