Cytokine Inducing Activities of Rhizobial and Mesorhizobial Lipopolysaccharides of Different Lethal Toxicity

• Published in: Immunobiology (1979) Vol. 202; no. 4; pp. 408 - 420
• Main Authors: Urbanik-Sypniewska, T., Choma, A., Kutkowska, J., KamiÑska, T., Kandefer-SzerszeÑ, M., Russa, R., Dolecka, J.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier GmbH 01.11.2000
• Subjects: Animals; g-Interferon; Interferon-gamma - biosynthesis; interleukin 1^b; interleukin 1b; Interleukin-1 - biosynthesis; Interleukin-6 - biosynthesis; Leukocytes - drug effects; Leukocytes - immunology; lipopolysaccharides; Lipopolysaccharides - immunology; Lipopolysaccharides - pharmacology; Mesorhizobium loti; Mice; Rhizobiaceae - immunology; Rhizobium leguminosarum; Rhizobium leguminosarum - immunology; Salmonella typhimurium; Salmonella typhimurium - immunology; Spleen - cytology; tumor necrosis factor-^a; Tumor Necrosis Factor-alpha - biosynthesis; GC-MS; IFN-γ; G1cN; G1cAN; 31P NMR; DAG; Ga1A; IL-6; LPS; ELISA; IL-1ß
• ISSN: 0171-2985; 1878-3279
• DOI: 10.1016/S0171-2985(00)80043-1

The lethality and cytokines-inducing activity of lipopolysaccharides (LPS) obtained from nodulating bacteria, Rhizobium leguminosarum and Mesorhizobium loti, were compared to those of Salmonella typhimurium LPSThe activity of Rleguminosarum LPS was almost comparable to Salmonella endotoxin in terms of lethality, Limulus lysate gelating activity and in vivo tumor necrosis factor-ά (TNF-ά), interleukin-1ß (IL-lß), interleukin-6 (IL-6) and interferon-γ (IFN-γ) induction capacityIn contrast to high lethal toxicity of Rhizobium LPS, the lethality of LPS isolated from Mesorhizobium loti was more than 10 3-fold lowerWeak lethality ofLPS from Mesorhizobium correlated with low capacity of this LPS to induce TNF-ά, IL-1ß, IL-6 and IFN-γ both in vivo and in vitro in murine splenocytesThe examined overall chemical composition of LPS indicates a considerable distinction in their lipid A regionsLipid A's obtained from Rleguminosarum and Mloti differed from their enterobacterial counterpart with respect to lipid A sugar backbone, its phosphate content as well as the type and distribution of hydrophobic acyl residuesThe relation of lipid A chemotype and bioactivity of LPS from the two Rhizobiaceae genera is discussed.