High-resolution simultaneous microscopy of refractive index and fluorescent intensity distributions by using localized surface plasmons

We propose a localized surface plasmon microscope that provides simultaneous imaging of refractive index and fluorescent intensity distributions. We show experimental images of fluorescent and transparent particles under circular pupil illumination to confirm simultaneous high-resolution imaging. Fu...

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Bibliographic Details
Published inApplied optics. Optical technology and biomedical optics Vol. 52; no. 14; p. 3324
Main Authors Terakado, Goro, Ning, Jun, Watanabe, Koyo, Kano, Hiroshi
Format Journal Article
LanguageEnglish
Published United States 10.05.2013
Subjects
Equipment Design
Equipment Failure Analysis
Microscopy, Fluorescence - instrumentation
Refractometry - instrumentation
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Fluorescence - instrumentation
Surface Plasmon Resonance - instrumentation
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Summary:We propose a localized surface plasmon microscope that provides simultaneous imaging of refractive index and fluorescent intensity distributions. We show experimental images of fluorescent and transparent particles under circular pupil illumination to confirm simultaneous high-resolution imaging. Furthermore, we investigate applicability of annular pupil illumination employing two axicons to improve energy efficiency in the fluorescent imaging and find that a brighter image is obtainable by maintaining high spatial resolution for both imaging modes.
ISSN:2155-3165
DOI:10.1364/ao.52.003324