Attenuated pressor responses to amino acids in the rostral ventrolateral medulla after swimming training in conscious rats

• Published in: Autonomic neuroscience Vol. 122; no. 1; pp. 21 - 28
• Main Authors: Martins-Pinge, Marli Cardoso, Becker, Lenice Kappes, Garcia, Martha Regina Luccizano, Zoccal, Daniel Breseguello, Neto, Renata Vasconcelos, Basso, Leonardo Salomão, de Souza, Hugo Celso Dutra, Lopes, Oswaldo Ubríaco
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 30.10.2005; Elsevier
• Subjects: Animals; Antihypertensive Agents - pharmacology; Biological and medical sciences; Blood pressure; Blood Pressure - drug effects; Cardiovascular System - drug effects; Consciousness; Dose-Response Relationship, Drug; Exercise training; Fundamental and applied biological sciences. Psychology; Glutamate receptors; Glutamic Acid - administration & dosage; Glycine - administration & dosage; Heart Rate - drug effects; Hypertension - physiopathology; Hypotension - physiopathology; Injections, Intraventricular; Male; Medulla Oblongata - drug effects; Medulla Oblongata - physiology; Microinjections; Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ; Physical Conditioning, Animal - physiology; Prazosin - pharmacology; Rats; Rats, Wistar; Swimming - physiology; Sympathetic nervous system; Tachycardia - physiopathology; Vertebrates: nervous system and sense organs; Glutamate receptors; Blood pressure; Exercise training; Sympathetic nervous system; Physical exercise; Rat; Rodentia; Central nervous system; Glutamate receptor; Encephalon; Vertebrata; Mammalia; Autonomic nervous system; Aminoacid; Animal; Medulla oblongata; Swimming; Hemodynamics
• ISSN: 1566-0702; 1872-7484
• DOI: 10.1016/j.autneu.2005.07.007

The cardiovascular effects of microinjection of the amino acids glutamate and glycine within the rostral ventrolateral medulla (RVLM) after swimming training (ST) in unrestrained awake rats were investigated. Unilateral microinjection of l-glutamate (5, 20 and 50 mM, in 100 nl) produced a dose dependent increase in mean arterial pressure (MAP) in control (C) (16 ± 5 mm Hg; 29 ± 6 mm Hg; 43 ± 6 mm Hg) and swim (SW) (1 ± 1 mm Hg; 16 ± 2 mm Hg; 25 ± 3 mm Hg) groups. However, the magnitude of this response was lower in the swim group. Prazosin injection produced hypotension and tachycardia in both groups (C = − 43 ± 3 mm Hg/98 ± 16 bpm; SW = − 61 ± 5 mm Hg/115 ± 32 bpm). In the SW group the hypotension caused by prazosin was greater compared to C group, but the tachycardia was not different between them. After prazosin, glutamate response in RVLM was blocked in both groups as well. When glycine (10 mM or 1 M, in 100 nl) were microinjected into the RVLM of C group we observed two different effects: decrease in MAP with the lower dose and an increase in MAP with the higher dose (10 mM = − 13 ± 2 mm Hg; 1 M = 47 ± 6 mm Hg). However, after ST the hypertensive response to glycine was blunted with no alterations in the hypotensive response (10 mM = − 14 ± 1 mm Hg; 1 M = 18 ± 4 mm Hg). These findings suggest that RVLM is involved in the modulation of the sympathetic outflow to the cardiovascular system during exercise training.