Analysis of Antibody Response to the Outer Surface Protein Family in Lyme Borreliosis Patients

• Published in: The Journal of infectious diseases Vol. 178; no. 6; pp. 1676 - 1683
• Main Authors: Batsford, Stephen, Rust, Christian, Neubert, Uwe
• Format: Journal Article
• Language: English
• Published: Chicago, IL University Chicago Press 01.12.1998; University of Chicago Press; Oxford University Press
• Subjects: Acrodermatitis - blood; Acrodermatitis - immunology; Acrodermatitis - microbiology; Analysis of the immune response. Humoral and cellular immunity; Animals; Antibodies; Antibodies, Bacterial - biosynthesis; Antibodies, Bacterial - blood; Antibody Formation; Antibody production; Antigens; Arthritis; Arthritis - blood; Arthritis - immunology; Arthritis - microbiology; Bacterial diseases; Bacterial Outer Membrane Proteins - immunology; Biological and medical sciences; Borrelia burgdorferi; Borrelia burgdorferi Group - genetics; Borrelia burgdorferi Group - immunology; Borrelia burgdorferi Group - isolation & purification; Borrelia infections; Enzyme-Linked Immunosorbent Assay; Erythema - blood; Erythema - immunology; Erythema - microbiology; Erythema multiforme; France; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Genotype; Genotypes; Germany; Human bacterial diseases; Humans; Immunobiology; Infections; Infectious diseases; Lyme disease; Lyme Disease - blood; Lyme Disease - immunology; Major Article; Medical sciences; Membrane proteins; Skin - microbiology; Ticks - immunology; Tropical bacterial diseases; United States; Europe; Germany; France; United States; Human; Lyme disease; Borrelia burgdorferi; Membrane protein; Immune response; Spirochaetaceae; Spirochaetales; Antibody; Borrelia infection; IgG; Genotype; Arthritis; Serology; Infection; Bacteriosis; Bacteria; Frequency; Serotype; Spirachaetosis
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1086/314477

Studies on frequencies of serum antibodies to outer surface proteins (Osps) in Lyme disease have produced conflicting results. Osp antigens (A, B, and C) enriched by butanol extraction, which aids band identification in immunoblotting, were used to test sera for IgG antibody to Osp antigens from Borrelia burgdorferi isolates from each subspecies (sensu stricto, afzelii, and garinii). Individual isolates were selected to include all five known European OspA genotypes. Of arthritis sera, 83% (n = 29), and of acrodermatitis chronica atrophicans sera, 81% (n = 26), recognized OspA, B, and/or C. Of erythema migrans sera, 23% recognized OspA and/or B and a further 15% OspC alone. Only 5 (6%) of 86 sera (4 arthritis, 1 acrodermatitis chronica atrophicans, 0 erythema migrans) recognized all five OspA phenotypes tested. Marked differences in the reactions of individual sera to the various Osp antigens were seen, which helps reveal the causes of discrepancies between previous reports.