Immobilization of antibodies on alginate-chitosan beads

• Published in: International journal of pharmaceutics Vol. 206; no. 1; pp. 23 - 34
• Main Authors: Albarghouthi, Methal, Fara, Dib Abu, Saleem, Mohamad, El-Thaher, Talal, Matalka, Khalid, Badwan, Adnan
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 25.09.2000; Elsevier
• Subjects: Alginate-chitosan; Alginates - metabolism; Animals; Anti-lisinopril IgG; Antibodies; Antibodies, Anti-Idiotypic - drug effects; Antibodies, Anti-Idiotypic - metabolism; Antihypertensive agents; Beads; Binding Sites, Antibody - drug effects; Biocompatible Materials - metabolism; Biological and medical sciences; Cardiovascular system; Cells, Immobilized - metabolism; Chitin - analogs & derivatives; Chitin - metabolism; Chitosan; Fixatives - pharmacology; Gels; General pharmacology; Glutaral - pharmacology; Hydrogen-Ion Concentration; Immobilization; Immunoglobulin G - drug effects; Immunoglobulin G - metabolism; Medical sciences; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Rabbits; Immobilization; Antibodies; Anti-lisinopril IgG; Alginate-chitosan; Beads; Lisinopril; Purification; Stability; Pharmaceutical technology; IgG; Alginates; Glutaral; Hapten; Storage stability; Dosage form; Covalent bond; Antihypertensive agent; Chitosan; Spherical particle
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/S0378-5173(00)00470-1

An anti-hapten IgG was covalently immobilized on glutaraldehyde-activated alginate-chitosan gel beads. The antibody immobilization efficiency was influenced by glutaraldehyde-bead reaction time, IgG concentration and pH. In addition, immobilization conditions such as glutaraldehyde and antibody concentrations influenced antibody hapten binding affinity. The immobilized IgG on the beads was stable and no reduction in the percent binding to hapten was noticed following 25 days of storage. It was concluded that antibodies could be successfully immobilized on alginate-chitosan gel beads. Such a system can be applied for the development of immunoaffinity purification and immunoassays.