Comparison of different liquid chromatography methods for the determination of corticosteroids in biological matrices

• Published in: Journal of Chromatography A Vol. 893; no. 1; pp. 55 - 67
• Main Authors: Stolker, A.A.M, Schwillens, P.L.W.J, van Ginkel, L.A, Brinkman, U.A.Th
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 29.09.2000; Elsevier
• Subjects: Adrenal Cortex Hormones - analysis; Adrenal Cortex Hormones - urine; Analysis; Animal Feed - analysis; Animal productions; Biological and medical sciences; Chromatography, Affinity - methods; Corticosteroids; Feed and pet food industries; Food industries; Fundamental and applied biological sciences. Psychology; General pharmacology; Mass Spectrometry; Medical sciences; Pharmacology. Drug treatments; Sensitivity and Specificity; Steroids; Terrestrial animal productions; Vertebrates; Solid-phase extraction; Corticosteroids; Steroids; Solid phase extraction; Biological fluid; Chemical analysis; HPLC chromatography; Veterinary medicine; Chemical enrichment; Ion trap; Sample preparation; Immunosorbent; Flumetasone; Adsorbent; Ungulata; Feed; Quantitative analysis; Trace analysis; Urine; Corticosteroid; Farming animal; Dexamethasone; Bovine; Steroid hormone; Coupled method; Vertebrata; Mammalia; Ultraviolet detector; Mass spectrometry MS/MS; Artiodactyla; Mass spectrometry
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(00)00725-1

Various extraction techniques can be combined with column liquid chromatography (LC) and ultraviolet (UV) or mass spectrometric (MS) detection for the determination of synthetic corticosteroids in biological matrices. Target analysis of low concentrations of 25 μg/kg of dexamethasone in feed can be performed by combining immunoaffinity chromatography (IAC) and LC with UV detection. A straightforward multi-analyte procedure is obtained by tandem solid-phase extraction (SPE) and subsequent LC–UV. However, the limits of detection for feed samples are then relatively poor, viz. 100 μg/kg. A multi-analyte method which meets modern demands of about 5 μg/kg detection limit requires one-step SPE combined with LC–MS analysis. As regards urine corticosteroids can be determined down to a level of 0.5 μg/l by either SPE–LC–MS–MS or SPE(IAC)–LC–MS.